A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM

Nicola Moscelli, Sander van den Driesche, Michael J. Vellekoop, Wojciech Witarski

2011

Abstract

In this contribution we present a compact imaging system to monitor the proliferation and the motility of cells in real-time. Our monitoring system is compatible with standard multi-well plates and operates in CO2, temperature and humidity controlled cell-culture incubators. Adherent grown epithelial cells in a multi-well plate well, positioned on top of a custom made holder, have been monitored in real-time with a fixed CCD image sensor. As light source an LED is placed above the plate holder. A field of view of 3.3 × 2.5 mm2 was achieved by using a 4.6 × 4.0 mm2 image sensor and mini lens system. The image sensor has a resolution of 640 × 480 pixels. Consequently, the obtained sensing resolution of the imaging system is about 5 μm. The cell monitoring system has first been validated by visualizing micro-beads of known dimensions. Then, our system has been successfully tested, tracking the migration paths and proliferation of respectively adherent grown MDCK (Madin-Darby canine kidney) and A549 (human lung carcinoma) epithelial cells.

References

  1. Suresh, S., 2007. Biomechanics and Biophysics of Cancer Cells. Acta Materialia, Vol. 55, Elsevier.
  2. Niinaka, Y., Haga, A., Raz, A., 2001. Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay. Methods in Molecular Medicine, Vol. 58, Springer.
  3. Poujade, M., Grasland-Mongrain, E., Hertzog, A., Jouanneau, J., Chavrier, P., Ladoux, B., Buguin, A., Silberzan, P., 2007. Collective Migration of an Epithelial Monolayer in Response to a Model Wound. PNAS, Vol. 104, No. 41.
  4. Gabriel, M., Picollet-D'hahan, N., Block, M., Haguet, V., Monitoring Adherent Cells by Contact Imaging, 2009. In Proc. MicroTAS 2009.
  5. Ji, H., Sander, D., Haas, A., Abshire, P. A., Contact Imaging: Simulation and Experiment, 2007. IEEE Trans Circ Sys, Vol. 54, No. 8.
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Paper Citation


in Harvard Style

Moscelli N., van den Driesche S., Vellekoop M. and Witarski W. (2011). A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 230-233. DOI: 10.5220/0003167002300233


in Bibtex Style

@conference{biodevices11,
author={Nicola Moscelli and Sander van den Driesche and Michael J. Vellekoop and Wojciech Witarski},
title={A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={230-233},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003167002300233},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM
SN - 978-989-8425-37-9
AU - Moscelli N.
AU - van den Driesche S.
AU - Vellekoop M.
AU - Witarski W.
PY - 2011
SP - 230
EP - 233
DO - 10.5220/0003167002300233