IN-SITU SPECTROSCOPIC INVESTIGATION OF UNFOLDING AND AGGREGATION OF INSULIN UNDER ULTRASONIC EXCITATION - An Ultrasonic Actuator for FTIR-spectrometry on Biomatter

Helge Pfeiffer, Nikos Chatziathanasiou, Filip Meersman, Christ Glorieux, Karel Heremans, Martine Wevers

2012

Abstract

It is well-known that fibrillogenesis of proteins can be influenced by diverse external parameters, such as temperature, pressure, agitation or chemical agents. This paper presents a newly developed ultrasonic actuator cell and a corresponding first feasibility study shows that also ultrasonic excitation at moderate intensities has a clear influence on the unfolding and aggregation behaviour of insulin. Irradiation with an average sound intensity of about 180 mW/cm2 leads to a decrease of the unfolding and aggregation temperature up to 7 K.

References

  1. Bolder, S. G., L. M. C. Sagis, et al. 2007. "Effect of stirring and seeding on whey protein fibril formation." Journal of Agricultural and Food Chemistry 55(14): 5661-5669.
  2. Bouchard, M., J. Zurdo, et al. 2000. "Formation of insulin amyloid fibrils followed by FTIR simultaneously with CD and electron microscopy." Protein Science 9(10): 1960-1967.
  3. Dobson, C. M. 2003. "Protein folding and misfolding." Nature 426(6968): 884-890.
  4. Grudzielanek, S., V. Smirnovas, et al. 2006. "Solvationassisted pressure tuning of insulin fibrillation: From novel aggregation pathways to biotechnological applications." Journal of Molecular Biology 356(2): 497-509.
  5. Suslick, K. S. (1988). Ultrasound: its chemical, physical, and biological effects. New York, N.Y., VCH Publishers.
  6. Tata, D. B. and F. Dunn. 1992. "Thermodynamic method of b/a analysis of liposome suspensions - prediction via single relaxation theory." Journal of Ultrasound in Medicine 11(8): 441-447.
  7. Wu, J. and W. L. M. Nyborg (2006). Emerging therapeutic ultrasound. Hackensack, N.J., World Scientific.
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Paper Citation


in Harvard Style

Pfeiffer H., Chatziathanasiou N., Meersman F., Glorieux C., Heremans K. and Wevers M. (2012). IN-SITU SPECTROSCOPIC INVESTIGATION OF UNFOLDING AND AGGREGATION OF INSULIN UNDER ULTRASONIC EXCITATION - An Ultrasonic Actuator for FTIR-spectrometry on Biomatter . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012) ISBN 978-989-8425-91-1, pages 372-375. DOI: 10.5220/0003797003720375


in Bibtex Style

@conference{biodevices12,
author={Helge Pfeiffer and Nikos Chatziathanasiou and Filip Meersman and Christ Glorieux and Karel Heremans and Martine Wevers},
title={IN-SITU SPECTROSCOPIC INVESTIGATION OF UNFOLDING AND AGGREGATION OF INSULIN UNDER ULTRASONIC EXCITATION - An Ultrasonic Actuator for FTIR-spectrometry on Biomatter},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)},
year={2012},
pages={372-375},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003797003720375},
isbn={978-989-8425-91-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)
TI - IN-SITU SPECTROSCOPIC INVESTIGATION OF UNFOLDING AND AGGREGATION OF INSULIN UNDER ULTRASONIC EXCITATION - An Ultrasonic Actuator for FTIR-spectrometry on Biomatter
SN - 978-989-8425-91-1
AU - Pfeiffer H.
AU - Chatziathanasiou N.
AU - Meersman F.
AU - Glorieux C.
AU - Heremans K.
AU - Wevers M.
PY - 2012
SP - 372
EP - 375
DO - 10.5220/0003797003720375