Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line

Hanna Sari Widya Kusuma, Hartini Tiono, Philips Onggowidjaja, Selonan Susang Obeng, Wahyu Widowati, Cintani Dewi Wahyuni, Cahyaning Riski Wijayanti, Muhamad Aldi Maulana, Tri Handayani, Rizal Rizal, Rizal Rizal

2021

Abstract

BACKGROUND: Inflammation is a biological response process by the immune system that may induce acute/chronic inflammatory and leading tissue damage or diseases. Pineapple (Ananas comosus) cores that have been investigated for anti-inflammatory properties and immunomodulator. OBJECTIVE: This research aims to evaluate the anti-inflammatory potency of pineapple core extract (PCE) in lipopolysaccharide-induced macrophage cells (RAW 264.7). METHODS: The viability assay of PCE was determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to ensure the safe and non-toxic concentration in RAW 264.7 cells. The pro-inflammatory induction of cells using 200 μL of lipopolysaccharide (LPS). Levels of PGE-2 and pro-inflammatory cytokines IL-1β and TNF-α levels were measured using enzyme-linked immunosorbent assay. RESULTS: PCE 4 and 20 µg/mL showed high viability (>90\%) with the values 95.03\% and 92.94\%, respectively. PCE 20 µg/mL showed the lower of PGE-2 and TNF-α levels (507.68 pg/mL; 345.90 pg/mL) compared to PCE 4 µg/mL (795.37 pg/mL; 474.19 pg/mL) and positive control (870.48 pg/mL; 581.71 pg/mL). In IL-1β level, PCE 20 µg/mL showed the lower (217.63 pg/mL) compared to PCE 4 pg/mL (350.78 pg/mL) and positive control (433.53 pg/mL).

Paper Citation

in Harvard Style

Kusuma H., Tiono H., Onggowidjaja P., Obeng S., Widowati W., Wahyuni C., Wijayanti C., Maulana M., Handayani T. and Rizal R. (2021). Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 58-64. DOI: 10.5220/0010744300003113

in Bibtex Style

@conference{ice-tes21,
author={Hanna Kusuma and Hartini Tiono and Philips Onggowidjaja and Selonan Obeng and Wahyu Widowati and Cintani Wahyuni and Cahyaning Wijayanti and Muhamad Maulana and Tri Handayani and Rizal Rizal},
title={Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={58-64},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010744300003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line
SN - 978-989-758-601-9
AU - Kusuma H.
AU - Tiono H.
AU - Onggowidjaja P.
AU - Obeng S.
AU - Widowati W.
AU - Wahyuni C.
AU - Wijayanti C.
AU - Maulana M.
AU - Handayani T.
AU - Rizal R.
PY - 2021
SP - 58
EP - 64
DO - 10.5220/0010744300003113