LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications

M. Scaramuzza, A. Ferrario, E. Pasqualotto, G. Rosati, A. De Toni, M. Quarta, A. Paccagnella, C. Reggiani

2012

Abstract

In this work we present the preliminary resulting measurements of an enzyme-based biosensor for the amperometric detection of lactic acid (LA). The sensor is based on low-cost gold electrodes on polymeric substrate. The redox catalytic enzyme used for analyte amperometric detection is lactate oxidase (LOx) from Pediococcus sp. This enzyme has been immobilized over electrodes surfaces by direct adsorption methodologies. Analysis of the enzyme-modified electrodes have been carried out by means of Electrochemical Impedance Spectroscopy (EIS) and with the development of an equivalent electrical model, in order to improve the adsorption process. Biosensors performance have been evaluated with Cyclic Voltammetry (CVM) measurements in different lactic acid solutions with concentrations from 1 M up to 300 mM. The lactate sensitivity of this disposable biosensor results in about 6.24 µA mM-1 cm-2.

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Paper Citation


in Harvard Style

Scaramuzza M., Ferrario A., Pasqualotto E., Rosati G., De Toni A., Quarta M., Paccagnella A. and Reggiani C. (2012). LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012) ISBN 978-989-8425-91-1, pages 380-383. DOI: 10.5220/0003867603800383


in Bibtex Style

@conference{biodevices12,
author={M. Scaramuzza and A. Ferrario and E. Pasqualotto and G. Rosati and A. De Toni and M. Quarta and A. Paccagnella and C. Reggiani},
title={LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)},
year={2012},
pages={380-383},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003867603800383},
isbn={978-989-8425-91-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)
TI - LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications
SN - 978-989-8425-91-1
AU - Scaramuzza M.
AU - Ferrario A.
AU - Pasqualotto E.
AU - Rosati G.
AU - De Toni A.
AU - Quarta M.
AU - Paccagnella A.
AU - Reggiani C.
PY - 2012
SP - 380
EP - 383
DO - 10.5220/0003867603800383