FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH

Peter Peer, Luis Galo Corzo

Abstract

Two-dimensional gel electrophoresis (2-DE) images show the expression levels of several hundred of proteins where each protein is represented as a blob shaped spot of grey level values. The spot detection, i.e. segmentation process has to be efficient as it is the first step in the gel processing. Such extraction of information is a very complex task. In this paper we propose a real time spot detector that is basically a morphology based method with use of seeded region growing as a central paradigm and which relies on the spot correlation information. The method is tested on gels with human samples in SWISS-2DPAGE (two-dimensional polyacrylamide gel electrophoresis) database. The average time to process the image is less than a second, while the results are very intuitive for human perception and as such they help the user to focus on important parts of the gel in the subsequent processing. In gels with less than 50 identified spots as proteins (proteins that compose a proteome) in the mentioned database, the algorithm detects all obvious spots.

Download


Paper Citation


in Harvard Style

Peer P. and Galo Corzo L. (2007). FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH . In Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, ISBN 978-972-8865-73-3, pages 284-289. DOI: 10.5220/0002050002840289


in Bibtex Style

@conference{visapp07,
author={Peter Peer and Luis Galo Corzo},
title={FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH},
booktitle={Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP,},
year={2007},
pages={284-289},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002050002840289},
isbn={978-972-8865-73-3},
}


in EndNote Style

TY - CONF
JO - Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP,
TI - FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH
SN - 978-972-8865-73-3
AU - Peer P.
AU - Galo Corzo L.
PY - 2007
SP - 284
EP - 289
DO - 10.5220/0002050002840289