YEAST ON A CHIP - Single-cell Analyses of MAPK Signaling Pathways in Saccharomyces Cerevisiae using Cell Chips

Min Cheol Park, Moon Kyu Kwak, Hye Sung Cho, Kahp Y. Suh, Jae Young Hur, Sang-Hyun Park

Abstract

The mitogen-activated protein kinase (MAPK) signaling pathways are essential for cell growth, cell differentiation and survival in eukaryotes. The MAPK signaling pathways transmit signals from the cell surface to nucleus. The mating and high osmolarity responses in the budding yeast, Saccharomyces cerevisiae, depend on the MAPK signaling pathways. Here we analyzed the mating and high osmolarity responses in the budding yeast, S. cerevisiae at single-cell level using cell chips. The cell chip analyses of the mating and high osmolarity responses were performed using fluorescent proteins fused to genes whose transcription is specifically upregulated by each signaling. Using the technique, we have determined the real-time gene expression patterns of the mating and high osmolarity responses at single-cell level. In this study, we observed that the mating and high osmolarity MAPK signaling showed a non-uniform, fluctuating flux in the population of yeast cells analyzed.

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Paper Citation


in Harvard Style

Cheol Park M., Kyu Kwak M., Sung Cho H., Y. Suh K., Young Hur J. and Park S. (2008). YEAST ON A CHIP - Single-cell Analyses of MAPK Signaling Pathways in Saccharomyces Cerevisiae using Cell Chips . In Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 2: BIODEVICES, (BIOSTEC 2008) ISBN 978-989-8111- 17-3, pages 268-271. DOI: 10.5220/0001050602680271


in Bibtex Style

@conference{biodevices08,
author={Min Cheol Park and Moon Kyu Kwak and Hye Sung Cho and Kahp Y. Suh and Jae Young Hur and Sang-Hyun Park},
title={YEAST ON A CHIP - Single-cell Analyses of MAPK Signaling Pathways in Saccharomyces Cerevisiae using Cell Chips},
booktitle={Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 2: BIODEVICES, (BIOSTEC 2008)},
year={2008},
pages={268-271},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001050602680271},
isbn={978-989-8111- 17-3},
}


in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 2: BIODEVICES, (BIOSTEC 2008)
TI - YEAST ON A CHIP - Single-cell Analyses of MAPK Signaling Pathways in Saccharomyces Cerevisiae using Cell Chips
SN - 978-989-8111- 17-3
AU - Cheol Park M.
AU - Kyu Kwak M.
AU - Sung Cho H.
AU - Y. Suh K.
AU - Young Hur J.
AU - Park S.
PY - 2008
SP - 268
EP - 271
DO - 10.5220/0001050602680271