RAPID FINITE STATE MACHINE CONTROL OF INDIVIDUAL DNA MOLECULES IN A NANOPORE

Noah A. Wilson, Robin Abu-Shumays, Elizabeth Koch, Seico Benner, William B. Dunbar

Abstract

This paper demonstrates feedback voltage control of individual DNA hairpin molecules captured in a nanopore. A finite state machine is used to program voltage control logic, executed on a field-programmable gate array, for rapid detection and regulation of hundreds of DNA hairpins, one at a time. Prompt voltage reduction is used for extension of the dwell time of DNA hairpins in the nanopore. Then, voltage reversal after a preset dwell time is used for automated expulsion of molecules prior to hairpin unzipping. The demonstrated control authority of single molecular complexes captured in the nanopore device is an integral part of our ongoing research for direct monitoring and control of enzyme-bound biopolymers.

Download


Paper Citation


in Harvard Style

A. Wilson N., Abu-Shumays R., Koch E., Benner S. and B. Dunbar W. (2008). RAPID FINITE STATE MACHINE CONTROL OF INDIVIDUAL DNA MOLECULES IN A NANOPORE . In Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 2: BIODEVICES, (BIOSTEC 2008) ISBN 978-989-8111- 17-3, pages 93-98. DOI: 10.5220/0001053000930098


in Bibtex Style

@conference{biodevices08,
author={Noah A. Wilson and Robin Abu-Shumays and Elizabeth Koch and Seico Benner and William B. Dunbar},
title={RAPID FINITE STATE MACHINE CONTROL OF INDIVIDUAL DNA MOLECULES IN A NANOPORE},
booktitle={Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 2: BIODEVICES, (BIOSTEC 2008)},
year={2008},
pages={93-98},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001053000930098},
isbn={978-989-8111- 17-3},
}


in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 2: BIODEVICES, (BIOSTEC 2008)
TI - RAPID FINITE STATE MACHINE CONTROL OF INDIVIDUAL DNA MOLECULES IN A NANOPORE
SN - 978-989-8111- 17-3
AU - A. Wilson N.
AU - Abu-Shumays R.
AU - Koch E.
AU - Benner S.
AU - B. Dunbar W.
PY - 2008
SP - 93
EP - 98
DO - 10.5220/0001053000930098