THERMORESPONSIVE POLYMER-BASED MICRODEVICE FOR NANO-LIQUID CHROMATOGRAPHY

Guillaume Paumier, Sovann Siv, Aurélien Bancaud, Jan Sudor, Anne-Marie Gué

Abstract

We report here on the development of an integrated device for sample desalting and pre-concentration for nanoLC / ESI-MS analysis combining poly-(N-isopropyl acrylamide) (PNIPAM) grafted microbeads and the means to dynamically control their temperature. Thermoresponsive properties of PNIPAM induce switchable hydrophobic/hydrophilic surfaces on which peptides can reversibly adsorb and desorb. The device is fabricated on a glass or pyrex substrate with deposited Ti/Au electrodes serving as built-in resistive heating sources. Pre-molded microfluidic channels and reservoirs made in PDMS are eventually assembled. Electrical and thermal characterization together with multiphysics modeling have been performed. The SiO2 surfaces of the channels and silica beads used as carriers of the stationary phases have been end-grafted with PNIPAM and employed to study the reversible adsorption and release kinetics of albumin-fluorescein conjugates by fluorescence video microscopy. It is clearly shown albumin-fluorescein complexes adsorb on beads surfaces above the transition temperature of PNIPAM (hydrophobic state), and are released when the temperature decreases (hydrophilic state), yet not fully reversibly.

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Paper Citation


in Harvard Style

Paumier G., Siv S., Bancaud A., Sudor J. and Gué A. (2008). THERMORESPONSIVE POLYMER-BASED MICRODEVICE FOR NANO-LIQUID CHROMATOGRAPHY . In Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2008) ISBN 978-989-8111- 17-3, pages 178-181. DOI: 10.5220/0001053101780181


in Bibtex Style

@conference{biodevices08,
author={Guillaume Paumier and Sovann Siv and Aurélien Bancaud and Jan Sudor and Anne-Marie Gué},
title={THERMORESPONSIVE POLYMER-BASED MICRODEVICE FOR NANO-LIQUID CHROMATOGRAPHY},
booktitle={Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2008)},
year={2008},
pages={178-181},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001053101780181},
isbn={978-989-8111- 17-3},
}


in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2008)
TI - THERMORESPONSIVE POLYMER-BASED MICRODEVICE FOR NANO-LIQUID CHROMATOGRAPHY
SN - 978-989-8111- 17-3
AU - Paumier G.
AU - Siv S.
AU - Bancaud A.
AU - Sudor J.
AU - Gué A.
PY - 2008
SP - 178
EP - 181
DO - 10.5220/0001053101780181