FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY

J. S. Silva, R. C. Martins, A. A. Vicente, J. A. Teixeira

Abstract

UV-VIS spectroscopy is a powerfull qualitative and quantitative technique used in analytical chemistry, which gives information about electronic transitions of electrons in molecular orbitals. As in UV-VIS spectra there is no direct information on characteristic organic groups, vibrational spectroscopy (e.g. infrared) has been preferred for biological applications. In this research, we try to use state-of-the-art fiber optics probes to obtain UV-VIS-SWNIR diffusive reflectance measurements of yeasts and bacteria colonies on plate count agar in the region of 200-1200nm; in order to discriminate the following microorganisms: i) yeasts: Saccharomyces cerevisiae, Saccharomyces bayanus, Candida albicans, Yarrowia lipolytica; and ii) bacteria: Micrococcus luteus, Pseudomonas fluorescens, Escherichia coli, Bacillus cereus. Spectroscopy results show that UV-VIS-SWNIR has great potential for identifying microorganisms on plate count agar. Scattering artifacts of both colonies and plate count agar can be significantly removed using a robust mean scattering algorithm, allowing also better discriminations between the scores obtained by singular value decomposition. Hierarchical clustering analysis of UV-VIS and VIS-SWNIR decomposed spectral scores lead to the conclusion that the use of VIS-SWNIR light source produces higher discrimination ratios for all the studied microorganisms, presenting great potential for developing biotechnology applications.

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Paper Citation


in Harvard Style

S. Silva J., C. Martins R., A. Vicente A. and A. Teixeira J. (2008). FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY . In Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2008) ISBN 978-989-8111-18-0, pages 25-32. DOI: 10.5220/0001062800250032


in Bibtex Style

@conference{biosignals08,
author={J. S. Silva and R. C. Martins and A. A. Vicente and J. A. Teixeira},
title={FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY},
booktitle={Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2008)},
year={2008},
pages={25-32},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001062800250032},
isbn={978-989-8111-18-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2008)
TI - FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY
SN - 978-989-8111-18-0
AU - S. Silva J.
AU - C. Martins R.
AU - A. Vicente A.
AU - A. Teixeira J.
PY - 2008
SP - 25
EP - 32
DO - 10.5220/0001062800250032