HAND-HELD LUMINOMETER WITH ECL-BASED BIOSENSOR FOR LACTATE DETERMINATION

A. Martínez-Olmos, A. J. Palma, J. Ballesta-Claver, M. C. Valencia-Miron, L. F. Capitan-Vallvey

Abstract

A new hand-held luminometer for electrochemiluminescence (ECL)-based one-shot biosensor for lactate is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of luminol, all reagents immobilized in a Methocel membrane. The measurement of ECL from a screenprinted electrode by a portable instrument designed and developed by the authors makes it possible to determine lactate concentration. The compositions of the membrane and reaction conditions have been optimized to obtain adequate sensitivity. The one-shot biosensor responds to lactate rapidly, with the typical ECL acquisition time being 3 min, with a linearized dependence whose dynamic range was from 9·10-6 to 2·10-3 M, a detection limit of 2.4·10-6 M and a sensor-to-sensor reproducibility (relative standard deviation RSD) around 10 % at the medium level of the range. The performance of the ECL one-shot biosensor and portable instrument was tested for the non invasive analysis of lactate in saliva, validating the results against a reference procedure.

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Paper Citation


in Harvard Style

Martínez-Olmos A., J. Palma A., Ballesta-Claver J., C. Valencia-Miron M. and F. Capitan-Vallvey L. (2009). HAND-HELD LUMINOMETER WITH ECL-BASED BIOSENSOR FOR LACTATE DETERMINATION . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009) ISBN 978-989-8111- 64-7, pages 343-346. DOI: 10.5220/0001432703430346


in Bibtex Style

@conference{biodevices09,
author={A. Martínez-Olmos and A. J. Palma and J. Ballesta-Claver and M. C. Valencia-Miron and L. F. Capitan-Vallvey},
title={HAND-HELD LUMINOMETER WITH ECL-BASED BIOSENSOR FOR LACTATE DETERMINATION},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)},
year={2009},
pages={343-346},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001432703430346},
isbn={978-989-8111- 64-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)
TI - HAND-HELD LUMINOMETER WITH ECL-BASED BIOSENSOR FOR LACTATE DETERMINATION
SN - 978-989-8111- 64-7
AU - Martínez-Olmos A.
AU - J. Palma A.
AU - Ballesta-Claver J.
AU - C. Valencia-Miron M.
AU - F. Capitan-Vallvey L.
PY - 2009
SP - 343
EP - 346
DO - 10.5220/0001432703430346