SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS

Bohuslav Rezek, Lenka Michalíková, Egor Ukraintsev, Alexander Kromka, Marie Kalbacova

Abstract

Unique combination of chemical and biocompatible properties with semiconducting properties makes diamond an attractive material for merging solid state and biological systems. Microscopic chemical patterning of diamond films by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly on the diamond is further controlled and optimized by cell and protein (fetal bovine serum - FBS) concentration. The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged into arrays on O-terminated patterns. The best cell selectivity is achieved for the lowest cell concentrations of 2500 cells/cm2. Higher cell concentrations enable to colonize unfavorable H-terminated regions due to mutual cell communication. Based on AFM, the proteins are present on both H- and O-terminated surfaces, however, pronounced differences in the thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and hence the cell selectivity. There is no cell selectivity when no protein is supplemented in the medium. These results may be applicable in tissue engineering, implants, bio-electronics, and biotechnology in general.

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Paper Citation


in Harvard Style

Rezek B., Michalíková L., Ukraintsev E., Kromka A. and Kalbacova M. (2009). SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009) ISBN 978-989-8111- 64-7, pages 347-354. DOI: 10.5220/0001434203470354


in Bibtex Style

@conference{biodevices09,
author={Bohuslav Rezek and Lenka Michalíková and Egor Ukraintsev and Alexander Kromka and Marie Kalbacova},
title={SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)},
year={2009},
pages={347-354},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001434203470354},
isbn={978-989-8111- 64-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)
TI - SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS
SN - 978-989-8111- 64-7
AU - Rezek B.
AU - Michalíková L.
AU - Ukraintsev E.
AU - Kromka A.
AU - Kalbacova M.
PY - 2009
SP - 347
EP - 354
DO - 10.5220/0001434203470354