A NOVEL MOBILE MONITORING SYSTEM FOR FAST AND AUTOMATED BACTERIA DETECTION IN WATER

Christoph Heller, Ulrich Reidt, Andreas Helwig, Florian Klettner, Gerhard Müller, Alois Friedberger, Leonhard Meixner, Karl Neumeier, Petra Lindner, Ramona Molz, Hans Wolf

Abstract

Standard detection methods for viable bacteria in potable water are time consuming due to a required cultivation step. Fast and automated detection of water borne microorganisms with high sensitivity and selectivity is still a challenge. We report on a novel biosensor using micromechanical filters with nano sized pores to capture and enrich bacteria on the filter surface and subsequent detection using fluorescent probes. The whole process is fully automated by integrating the sieves into a fluidic system together with a high performance fluorescence detector. The results show the effective retention of bacteria on the filter surface, which are then accessible for different staining procedures. As an example, we use special fluorescent dyes that bind to or intercalate in the DNA molecules of the bacteria. After detection, the microfilters undergo cleaning and conditioning steps to be ready for the next measurement.

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Paper Citation


in Harvard Style

Heller C., Reidt U., Helwig A., Klettner F., Müller G., Friedberger A., Meixner L., Neumeier K., Lindner P., Molz R. and Wolf H. (2009). A NOVEL MOBILE MONITORING SYSTEM FOR FAST AND AUTOMATED BACTERIA DETECTION IN WATER . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009) ISBN 978-989-8111- 64-7, pages 384-389. DOI: 10.5220/0001545203840389


in Bibtex Style

@conference{biodevices09,
author={Christoph Heller and Ulrich Reidt and Andreas Helwig and Florian Klettner and Gerhard Müller and Alois Friedberger and Leonhard Meixner and Karl Neumeier and Petra Lindner and Ramona Molz and Hans Wolf},
title={A NOVEL MOBILE MONITORING SYSTEM FOR FAST AND AUTOMATED BACTERIA DETECTION IN WATER},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)},
year={2009},
pages={384-389},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001545203840389},
isbn={978-989-8111- 64-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)
TI - A NOVEL MOBILE MONITORING SYSTEM FOR FAST AND AUTOMATED BACTERIA DETECTION IN WATER
SN - 978-989-8111- 64-7
AU - Heller C.
AU - Reidt U.
AU - Helwig A.
AU - Klettner F.
AU - Müller G.
AU - Friedberger A.
AU - Meixner L.
AU - Neumeier K.
AU - Lindner P.
AU - Molz R.
AU - Wolf H.
PY - 2009
SP - 384
EP - 389
DO - 10.5220/0001545203840389