CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES

Kuan Yan, Fons Verbeek, Sylvia Le Dévédec, Bob van de Water

Abstract

In this paper, we address the problem of the analysis of cellular phenotype from time-lapse image sequences using object tracking algorithms and feature extraction and classification. We discusses the application of an object tracking algorithm for in the analysis of high content cell-migration time-lapse image sequence of extremely motile cells; these cells are captured at low time-resolution.. The small size of the objects and significant deformation of the object during the process renders the tracking as a non-trivial problem. To that end, the ‘KDE Mean Shift’, a real-time tracking solution, is adapted for our research. We illustrate that in a simulation experiment with artificial objects, with our algorithm an accuracy of over 90% can be established. Based on the tracking result, we propose several morphology and motility based measurements for the analysis of cell behaviour. Our analysis requires only initial manual interference; the majority of the processing is automated.

References

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Paper Citation


in Harvard Style

Yan K., Verbeek F., Le Dévédec S. and van de Water B. (2009). CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES . In Proceedings of the Fourth International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, (VISIGRAPP 2009) ISBN 978-989-8111-69-2, pages 281-286. DOI: 10.5220/0001804302810286


in Bibtex Style

@conference{visapp09,
author={Kuan Yan and Fons Verbeek and Sylvia Le Dévédec and Bob van de Water},
title={CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES },
booktitle={Proceedings of the Fourth International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, (VISIGRAPP 2009)},
year={2009},
pages={281-286},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001804302810286},
isbn={978-989-8111-69-2},
}


in EndNote Style

TY - CONF
JO - Proceedings of the Fourth International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, (VISIGRAPP 2009)
TI - CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES
SN - 978-989-8111-69-2
AU - Yan K.
AU - Verbeek F.
AU - Le Dévédec S.
AU - van de Water B.
PY - 2009
SP - 281
EP - 286
DO - 10.5220/0001804302810286