3D Segmentation for the Study of Cell Cycle Progression in Live Drosophila Embryos

Chinta Rambabu, Puah Wee Choo, Janos Kriston-Vizi, Martin Waser

Abstract

We study the dynamics of cell division in live Drosophila embryos using fluorescent proteins and 3D time-lapse microscopy. Accurate segmentation of nuclei and mitotic chromosomes labeled by the live reporter histone-GFP is a prerequisite for subsequent tracking and quantitative object analysis. We propose an automated 3D segmentation method based on narrow band level sets that preserves the boundary of the cell nuclei and removes signals that are artifacts of live cell imaging. We introduce an improved 3D narrow band approach in the region shrinking and growing process for accurately segmenting the cell nuclei from background. The proposed method has been evaluated with the ground truth regarding the object level accuracy and segmentation quality. Both the object level accuracy and pixel accuracy of the proposed method are around 96% and 85% respectively. Our algorithm can robustly segment nuclei and chromosomes in different phase of the division cycle.

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Paper Citation


in Harvard Style

Rambabu C., Wee Choo P., Kriston-Vizi J. and Waser M. (2009). 3D Segmentation for the Study of Cell Cycle Progression in Live Drosophila Embryos . In Proceedings of the 1st International Workshop on Medical Image Analysis and Description for Diagnosis Systems - Volume 1: Workshop MIAD, (BIOSTEC 2009) ISBN 978-989-8111-77-7, pages 43-51. DOI: 10.5220/0001813300430051


in Bibtex Style

@conference{workshop miad09,
author={Chinta Rambabu and Puah Wee Choo and Janos Kriston-Vizi and Martin Waser},
title={3D Segmentation for the Study of Cell Cycle Progression in Live Drosophila Embryos},
booktitle={Proceedings of the 1st International Workshop on Medical Image Analysis and Description for Diagnosis Systems - Volume 1: Workshop MIAD, (BIOSTEC 2009)},
year={2009},
pages={43-51},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001813300430051},
isbn={978-989-8111-77-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 1st International Workshop on Medical Image Analysis and Description for Diagnosis Systems - Volume 1: Workshop MIAD, (BIOSTEC 2009)
TI - 3D Segmentation for the Study of Cell Cycle Progression in Live Drosophila Embryos
SN - 978-989-8111-77-7
AU - Rambabu C.
AU - Wee Choo P.
AU - Kriston-Vizi J.
AU - Waser M.
PY - 2009
SP - 43
EP - 51
DO - 10.5220/0001813300430051