A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images

I. Gurevich, I. Koryabkina, E. Kozina, A. Myagkov, H. Niemann, M. Ugrumov, V. Yashina

Abstract

This work is devoted to the description of an experimental data acquisition automated method which is required to fill the model of Parkinson’s disease preclinical stage. Digital images of the immunostained brain sections of experimental animals are used as a data source. Proposed method: 1) is based on following mathematical morphology operations: opening, grayscale reconstruction, closing, bot-hat transformation, morphological gradient, watershed transformation; 2) enables: to smooth heterogeneous complex background; to select small objects on images depended on given sizes and gray values; to eliminate out-of-focus objects; to separate close objects; to calculate features of selected objects; 3) is intended for automatic extraction of dopaminergic neurons terminals on striatum frontal section images. Experimental investigations confirmed possibility and suitability of section images automated processing and analysis by means of the method. The results of the method use are segmented object contours binary image and object feature list.

References

  1. Gurevich, I.B., Yashina, V.V., Koryabkina, I.V., Niemann, H., Salvetti, O.: Descriptive approach to medical image mining: An algorithmic scheme for analysis of cytological specimens. Pattern Recognition and Image Analysis: Advances in Mathematical Theory and Applications 18 (2008) 542-562
  2. Gurevich, I., Harazishvili, D., Jernova, I., et al.: Information technology for the morphological analysis of the lymphoid cell nuclei. In: The 13th Scandinavian Conference on Image Analysis. Volume 2749 of LNCS. (2003) 541-548
  3. Albin, R.L., Young, A.B., Penney, J.B.: The functional anatomy of basal ganglia disorders. Trends Neurosci 12 (1989) 366-75
  4. Tipton, K.F., Singer, T.P.: Advances in our understanding of the mechanisms of the neurotoxicity of mptp and related compounds. J Neurochem 61 (1993) 1191-1206
  5. Vincent, L.: Morphological grayscale reconstruction in image analysis: Applications and efficient algorithms. IEEE Transactions on Image Processing 2 (1993) 176-201
  6. Soille, P.: Morphological Image Analysis: Principles and Applications. Springer, Berlin (2004)
  7. Wu, Q., Merchant, F., Castleman, K.: MICROSCOPE IMAGE PROCESSING. Elsevier Inc. (2008)
  8. Gonsales, R.C., Woods, R.E.: Digital Image Processing. 2 edn. Pearson Education, Inc (2002) publishing as Prentice Hall.
  9. Vincent, L., Soille, P.: Watersheds in digital spaces: an efficient algorithm based on immersion simulations. IEEE Trans. Pattern Anal. Machine Intell. 6 (1991) 583-598
Download


Paper Citation


in Harvard Style

Gurevich I., Koryabkina I., Kozina E., Myagkov A., Niemann H., Ugrumov M. and Yashina V. (2009). A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images . In Proceedings of the 2nd International Workshop on Image Mining Theory and Applications - Volume 1: Workshop IMTA, (VISIGRAPP 2009) ISBN 978-989-8111-80-7, pages 30-39. DOI: 10.5220/0001964600300039


in Bibtex Style

@conference{workshop imta09,
author={I. Gurevich and I. Koryabkina and E. Kozina and A. Myagkov and H. Niemann and M. Ugrumov and V. Yashina},
title={A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images},
booktitle={Proceedings of the 2nd International Workshop on Image Mining Theory and Applications - Volume 1: Workshop IMTA, (VISIGRAPP 2009)},
year={2009},
pages={30-39},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001964600300039},
isbn={978-989-8111-80-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 2nd International Workshop on Image Mining Theory and Applications - Volume 1: Workshop IMTA, (VISIGRAPP 2009)
TI - A Method for Automatic Extraction of Dopaminergic Neuron Terminals on Striatum Frontal Section Images
SN - 978-989-8111-80-7
AU - Gurevich I.
AU - Koryabkina I.
AU - Kozina E.
AU - Myagkov A.
AU - Niemann H.
AU - Ugrumov M.
AU - Yashina V.
PY - 2009
SP - 30
EP - 39
DO - 10.5220/0001964600300039