SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION

Alessandro Giusti, Giorgio Corani, Luca Gambardella, Cristina Magli, Luca Gianaroli

Abstract

Observation of ovocytes and zygotes plays an important role in In Vitro Fertilization procedures, and is usually perfomed by means of a microscope equipped with Hoffman Modulation Contrast optics, which produces images with a complex, side-lit, 3D-like appearance. Our algorithm operates on a Z-stack of such images taken at different focal planes, and simultaneously identifies: a) a repeatable, meaningful focal plane corresponding to the cell's equator line, and b) the external contour of the cell. As the cell is a thick stucture with respect to the microscope depth of field, the two problems are nontrivial and deeply related. Our algorithm is also robust to other structures, clutter and artefacts affecting the images and lying at varying focal planes. We describe implementation details, applications and experimental results of our technique.

References

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Paper Citation


in Harvard Style

Giusti A., Corani G., Gambardella L., Magli C. and Gianaroli L. (2010). SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION . In Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010) ISBN 978-989-674-018-4, pages 151-158. DOI: 10.5220/0002592501510158


in Bibtex Style

@conference{biosignals10,
author={Alessandro Giusti and Giorgio Corani and Luca Gambardella and Cristina Magli and Luca Gianaroli},
title={SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION},
booktitle={Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010)},
year={2010},
pages={151-158},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002592501510158},
isbn={978-989-674-018-4},
}


in EndNote Style

TY - CONF
JO - Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010)
TI - SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION
SN - 978-989-674-018-4
AU - Giusti A.
AU - Corani G.
AU - Gambardella L.
AU - Magli C.
AU - Gianaroli L.
PY - 2010
SP - 151
EP - 158
DO - 10.5220/0002592501510158