SYSTEMATIC POSITION AND PHYLOGENETIC RELATIONSHIPS OF THE CYCLOPHYLLIDEAN CESTODES - An In-silico Study using ITS2 rDNA and Sequence-structure Alignment

Veena Tandon, Devendra Kumar Biswal, Pramod Kumar Prasad, Chenkual Malsawmtluangi

Abstract

The phylogenetic relationships and systematic position of cyclophyllidean cestodes have always been controversial and opinions of different authors on the systematic rank and content of this order have varied greatly. Molecular phylogenetic analysis based on ITS2 rDNA of 16 representatives spanning 6 different families (Mesocestoididae, Davaineidae, Anoplocephalidae, Taeniidae, Dipylidiidae and Hymenolepididae) of the Order Cyclophyllidea and one out group from the family Diphyllobothriidae of the Order Pseudophyllidea confirmed the monophyletic nature of the Order Cyclophyllidea. Further, the results were validated by bayesian analysis, primary sequence-structure alignment and subsequent molecular morphometrics analysis. At the major nodes all the trees from various analyses were similar. Mesocestoides was interestingly accommodated within Cyclophyllidea and served as a sister clade close to the families Taeniidae, Anoplocephalidae, Hymenolepididae and Dipylidiidae.

References

  1. Billoud, B., Guerrucci, M. A., Masselot, M., Deutsch J.S., 2000. Cirripede phylogeny using a novel approach: molecular morphometrics. Molecular Biology and Evolution, 17(10) pp. 1435-1445.
  2. Brooks, D. R., Hoberg, E. P., Weekes, P. J., 1991. Preliminary phylogenetic systematic analysis of the major lineages of the Eucestoda (Platyhelminthes: Cercomeria). Proceedings of the Biological Society of Washington, 104, pp. 651-688
  3. Chandler, A. C., 1946. Observations on the anatomy of Mesocestoides. Journal of Parasitology, 32, pp. 242- 246.
  4. Coleman, A.W., 2003. ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends in Genetics, 19(7), pp.370-375.
  5. Cote, C. A., Peculis, B. A., 2001. Role of the ITS 2- proximal stem and evidence for indirect recognition of processing sites in pre-rRNA processing in yeast. Nucleic Acids Research, 29, pp. 2106-2116.
  6. Dayhoff, M . O., Eck, R. V., Chang, M . A., Sochard, M. R., 1965. Atlas of Protein Sequence and Structure. Silver Spring, Maryland: National Biomedical Research Foundation
  7. Kamegai, S., Ichihara, A., Nonobe, H., Machida, M., 1967. The 6th and 7th records of human infection with Mesocestoides lineatus (Cestoda) in Japan. Research Bulletin of the Meguro Parasitological Museum, 1, pp. 1-7.
  8. Khalil, L. F., Jones, A., Bray, R. A. eds., 1994. Keys to the cestode parasites of vertebrates. Wallingford: CAB International.
  9. Mariaux, J., 1996. Cestode systematics: Any progress? International Journal for Parasitology, 26(3), pp. 231-243.
  10. Nei, M., Kumar, S., 2000. Molecular Evolution and Phylogenetics. New York: Oxford University Press.
  11. Page, R.D., 1996. TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12, pp. 357-358.
  12. Ronquist, F., Huelsenbeck, J. F., 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics,19, pp. 1572-1574.
  13. Saitou, N., Nei, M., 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, pp. 406-425.
  14. Schultz, J., Maisel, S., Gerlach, D., Müller, T., Wolf, M., 2005. A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota. RNA, 11(4), pp. 361-364.
  15. Siebert, S., Backofen, R., 2005. MARNA:multiple alignment and consensus structure prediction of RNAs based on sequence structure comparisons. Bioinformatics, 21, pp. 3352-3359
  16. Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology & Evolution, 24 (8), pp.1596-1599.
  17. Tamura, K., Dudley, J., Nei, M., Kumar, S., 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences,101, pp.11030-11035.
  18. Thompson, J. D., Higgins, D. G., Gibson, T. J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, (22), pp. 4673-80.
  19. von Nickisch-Rosenegk, M., Lucius, R., Loos-Frank, B., 1999. Contributions to the phylogeny of the Cyclophyllidea (Cestoda) inferred from mitochondrial 12S rDNA. Journal of Molecular Evolution, 48(5), pp. 586-596.
  20. Wolf, M., Ruderisch, B., Dandekar, T., Schultz, J., Müller, T., 2008. ProfDistS: (profile-) distance based phylogeny on sequence--structure alignments. Bioinformatics, 24(20), pp.2401-2402.
  21. Zuker, M., 2003. Mfold web server for nucleic folding and hybridization prediction. Nucleic Acids Research, 31, pp. 3406-3415.
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Paper Citation


in Harvard Style

Tandon V., Kumar Biswal D., Kumar Prasad P. and Malsawmtluangi C. (2010). SYSTEMATIC POSITION AND PHYLOGENETIC RELATIONSHIPS OF THE CYCLOPHYLLIDEAN CESTODES - An In-silico Study using ITS2 rDNA and Sequence-structure Alignment . In Proceedings of the First International Conference on Bioinformatics - Volume 1: BIOINFORMATICS, (BIOSTEC 2010) ISBN 978-989-674-019-1, pages 5-12. DOI: 10.5220/0002690500050012


in Bibtex Style

@conference{bioinformatics10,
author={Veena Tandon and Devendra Kumar Biswal and Pramod Kumar Prasad and Chenkual Malsawmtluangi},
title={SYSTEMATIC POSITION AND PHYLOGENETIC RELATIONSHIPS OF THE CYCLOPHYLLIDEAN CESTODES - An In-silico Study using ITS2 rDNA and Sequence-structure Alignment},
booktitle={Proceedings of the First International Conference on Bioinformatics - Volume 1: BIOINFORMATICS, (BIOSTEC 2010)},
year={2010},
pages={5-12},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002690500050012},
isbn={978-989-674-019-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Bioinformatics - Volume 1: BIOINFORMATICS, (BIOSTEC 2010)
TI - SYSTEMATIC POSITION AND PHYLOGENETIC RELATIONSHIPS OF THE CYCLOPHYLLIDEAN CESTODES - An In-silico Study using ITS2 rDNA and Sequence-structure Alignment
SN - 978-989-674-019-1
AU - Tandon V.
AU - Kumar Biswal D.
AU - Kumar Prasad P.
AU - Malsawmtluangi C.
PY - 2010
SP - 5
EP - 12
DO - 10.5220/0002690500050012