BIOREACTOR FOR MECHANICAL CELL STIMULATION - Concept and Design

J. G. Rocha, V. Correia, J. L. Gomez Ribelles, S. Lanceros-Mendez, A. Pitaes

Abstract

Mechanical stimulation plays an important role in improving cell growth in the skeletal system, for example. In this article we describe a bioreactor in which cells in a three-dimensional scaffolds are stimulated by cyclically applied mechanical loads. The objective of this study is to develop a custom-designed bioreactor capable of applying controlled compressive loads to a cell-encapsulating scaffold. Its working principle is based on an innovative design of a feedback controlled electromagnetic actuator, which allows the application of compressive forces to the samples and at the same time, it allows the measurement of the produced displacement.

References

  1. Lee, S.J., Jeong Hun Park, Yong-Joon Seol, In Hwan Lee, Sang Soon Kang, Dong-Woo Cho, Development of a bioreactor for the mechanical stimulation of agarose hydrogels, Microelectronic Engineering 86 (2009) 1411-1415.
  2. Nugent-Derfus G. E., Takara T., O'Neilly J. K., Cahill S. B., Gortz S., Pong T., Inoue H., Aneloski N. M., Wang W. W., Vega K. I., Klein T. J., Hsieh-Bonassera N. D., Bae W. C., Burke J. D., Bugbee W. D. and Sah R. L., Continuous passive motion applied to whole joints stimulates chondrocyte biosynthesis of PRG4,OsteoArthritis and Cartilage (2007) 15, 566- 574.
  3. Kisidaya J. D., Jinb M., DiMiccod M. A., Kurze B.,Grodzinsky A. J., Effects of dynamic compressive loading on chondrocyte biosynthesis in selfassembling peptide scaffolds, Journal of Biomechanics 37 (2004) 595-604.
  4. Cooper Jr. J. A., Wan-Ju Li, LeeAnn O. Bailey, Steve D. Hudson, Sheng Lin-Gibson, Kristi S. Anseth, Rocky S. Tuan, Newell R. Washburn, Encapsulated chondrocyte response in a pulsatile flow bioreactor, Acta Biomaterialia 3 (2007) 13-21.
  5. Garvin, J., Qi, J., Maloney, M., Banes, A.J., Novel System for Engineering Bioartificial Tendons and Application of Mechanical Load, Tissue Engineering Vol. 9 (5) 2003, 967-979.
  6. Meyer U, Terodde M, Joos U, Wiesmann HP., Mechanical stimulation of osteoblasts in cell culture, Mund Kiefer Gesichtschir. 2001;5:166-72.
  7. Guldberg RE., Consideration of mechanical factors, Ann N Y Acad Sci 2002, 961, 312-314.
  8. Godstein AS, Juarez TM, Helmke CD, Gustin MC, Mikos AG. Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds. Biomaterials 2001, 22, 1279-1288.
  9. Botchwey EA, Pollack SR, Levine EM, Laurencin CT. Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system, J Biomed Mater Res 2001, 55, 242-253.
  10. Hillsley MV, Frangos JA. Review: Bone tissue engineering: the role of interstitial fluid flow, Biotechnol Bioeng 1994, 43, 573-581.
Download


Paper Citation


in Harvard Style

Rocha J., Correia V., Gomez Ribelles J., Lanceros-Mendez S. and Pitaes A. (2010). BIOREACTOR FOR MECHANICAL CELL STIMULATION - Concept and Design . In Proceedings of the Third International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2010) ISBN 978-989-674-017-7, pages 147-151. DOI: 10.5220/0002759401470151


in Bibtex Style

@conference{biodevices10,
author={J. G. Rocha and V. Correia and J. L. Gomez Ribelles and S. Lanceros-Mendez and A. Pitaes},
title={BIOREACTOR FOR MECHANICAL CELL STIMULATION - Concept and Design},
booktitle={Proceedings of the Third International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2010)},
year={2010},
pages={147-151},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002759401470151},
isbn={978-989-674-017-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the Third International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2010)
TI - BIOREACTOR FOR MECHANICAL CELL STIMULATION - Concept and Design
SN - 978-989-674-017-7
AU - Rocha J.
AU - Correia V.
AU - Gomez Ribelles J.
AU - Lanceros-Mendez S.
AU - Pitaes A.
PY - 2010
SP - 147
EP - 151
DO - 10.5220/0002759401470151