IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies

Evgeny O. Puchkov

Abstract

For Saccharomyces cerevisiae yeast studies, three approaches have been developed. They are based on the image analysis (ImageJ software) application for the fluorescence microscopy data treatment. The first is a computer-aided fluorescence microscopy procedure for quantifying of the damaged cells in the ethanol-producing yeast culture. It was shown to be applicable for the assessment of the culture viability. The second is a means of characterizing Brownian motion of the insoluble polyphosphate complexes in the vacuoles. Using this approach, the apparent viscosity in the vacuoles was measured. The third is a method for locating intracellular sites/targets of the nucleic acid intercalators. This method may be of help in designing of new DNA-targeted drugs and in preliminary studies of their interaction with eukaryotic cells.

References

  1. Puchkov, E. O., 2006. 'Viability assessment of ethanol-producing yeast by computer-aided fluorescence microscopy', Microbiology, vol. 75, no. 2, pp. 154-160.
  2. Puchkov, E. O., 2010. 'Brownian motion of polyphosphate complexes in yeast vacuoles: characterization by fluorescence microscopy with image analysis', Yeast, vol. 27, no. 6, pp. 309-315.
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Paper Citation


in Harvard Style

O. Puchkov E. (2011). IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies . In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011) ISBN 978-989-8425-36-2, pages 258-261. DOI: 10.5220/0003120902580261


in Bibtex Style

@conference{bioinformatics11,
author={Evgeny O. Puchkov},
title={IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)},
year={2011},
pages={258-261},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003120902580261},
isbn={978-989-8425-36-2},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)
TI - IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies
SN - 978-989-8425-36-2
AU - O. Puchkov E.
PY - 2011
SP - 258
EP - 261
DO - 10.5220/0003120902580261