TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY

Peter D. Dowd, Jan Karolin, Carol Trager-Cowan, David J. S. Birch, William H. Stimson

Abstract

A new method for detecting gonadotropin-releasing hormone type 1 (GnRH-1) is proposed. The method is based on a fluorescence polarisation immunoassay where the GnRH-1 vies for the binding site on the anti-GnRH (antibody 7B10.1D10) with a labelled peptide consisting of a fragment of GnRH labelled with fluorescein. Time-resolved anisotropy decays indicate increased correlation times for the labelled fragment in the presence of 7B10.1D10 compared with the correlation times for the labelled peptide fragment alone. When GnRH-1 is added to the solution of the labelled peptide in the presence of 7B10.1D10 it has the effect of reducing the correlation times. The results demonstrate the possibility of introducing the labelled fragment as a competitor to the antigen in the fluorescence polarisation immunoassay for GnRH-1 and, more generally, in the case where there is a disadvantage in labelling the antigen, such as GnRH-1, with a fluorescent probe.

References

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Paper Citation


in Harvard Style

Dowd P., Karolin J., Trager-Cowan C., S. Birch D. and Stimson W. (2011). TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 184-188. DOI: 10.5220/0003152001840188


in Bibtex Style

@conference{biodevices11,
author={Peter D. Dowd and Jan Karolin and Carol Trager-Cowan and David J. S. Birch and William H. Stimson},
title={TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={184-188},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003152001840188},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - TOWARDS A NEW HOMOGENEOUS IMMUNOASSAY FOR GONADOTROPIN-RELEASING HORMONE BASED ON TIME-RESOLVED FLUORESCENCE ANISOTROPY
SN - 978-989-8425-37-9
AU - Dowd P.
AU - Karolin J.
AU - Trager-Cowan C.
AU - S. Birch D.
AU - Stimson W.
PY - 2011
SP - 184
EP - 188
DO - 10.5220/0003152001840188