A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM

Nicola Moscelli, Sander van den Driesche, Michael J. Vellekoop, Wojciech Witarski

Abstract

In this contribution we present a compact imaging system to monitor the proliferation and the motility of cells in real-time. Our monitoring system is compatible with standard multi-well plates and operates in CO2, temperature and humidity controlled cell-culture incubators. Adherent grown epithelial cells in a multi-well plate well, positioned on top of a custom made holder, have been monitored in real-time with a fixed CCD image sensor. As light source an LED is placed above the plate holder. A field of view of 3.3 × 2.5 mm2 was achieved by using a 4.6 × 4.0 mm2 image sensor and mini lens system. The image sensor has a resolution of 640 × 480 pixels. Consequently, the obtained sensing resolution of the imaging system is about 5 μm. The cell monitoring system has first been validated by visualizing micro-beads of known dimensions. Then, our system has been successfully tested, tracking the migration paths and proliferation of respectively adherent grown MDCK (Madin-Darby canine kidney) and A549 (human lung carcinoma) epithelial cells.

References

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Paper Citation


in Harvard Style

Moscelli N., van den Driesche S., Vellekoop M. and Witarski W. (2011). A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 230-233. DOI: 10.5220/0003167002300233


in Bibtex Style

@conference{biodevices11,
author={Nicola Moscelli and Sander van den Driesche and Michael J. Vellekoop and Wojciech Witarski},
title={A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={230-233},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003167002300233},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM
SN - 978-989-8425-37-9
AU - Moscelli N.
AU - van den Driesche S.
AU - Vellekoop M.
AU - Witarski W.
PY - 2011
SP - 230
EP - 233
DO - 10.5220/0003167002300233