GAST, A GENOMIC ALIGNMENT SEARCH TOOL

Kalle Karhu, Juho Mäkinen, Jussi Rautio, Jorma Tarhio, Hugh Salamon

Abstract

Alignment to a genomic sequence is a common task in modern bioinformatics. By improving the methods used, significant amount of time and resources can be saved. We have developed a new genomic alignment search tool, called GAST, for sequences of at least 160 nt. GAST is many times faster than commonly used alignment tools BLAT and Mega BLAST. As the sizes of query sequences and the database increase, the advantage grows. This paper describes the principles of GAST and reports a comparison of GAST with BLAT and Mega BLAST. The effects the query sequence length and the number of queries have on run times were studied using the full human genome and the chromosome 1 of human genome separately. Additionally, the error tolerance and behaviour of GAST when handling sequences with lower similarity to a database was studied. Lastly, we compared the quality of exon mappings produced by the three tools and the genomic mapping tool GMAP.

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Paper Citation


in Harvard Style

Karhu K., Mäkinen J., Rautio J., Tarhio J. and Salamon H. (2011). GAST, A GENOMIC ALIGNMENT SEARCH TOOL . In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011) ISBN 978-989-8425-36-2, pages 82-90. DOI: 10.5220/0003181400820090


in Bibtex Style

@conference{bioinformatics11,
author={Kalle Karhu and Juho Mäkinen and Jussi Rautio and Jorma Tarhio and Hugh Salamon},
title={GAST, A GENOMIC ALIGNMENT SEARCH TOOL},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)},
year={2011},
pages={82-90},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003181400820090},
isbn={978-989-8425-36-2},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)
TI - GAST, A GENOMIC ALIGNMENT SEARCH TOOL
SN - 978-989-8425-36-2
AU - Karhu K.
AU - Mäkinen J.
AU - Rautio J.
AU - Tarhio J.
AU - Salamon H.
PY - 2011
SP - 82
EP - 90
DO - 10.5220/0003181400820090