TEXTURE ANALYSIS OF MILK PROTEIN GELS USING DIGITAL IMAGE ANALYSIS

Juan Pablo Costa, Horacio Castellini, Patricia Risso, Bibiana Riquelme

Abstract

Sodium caseinate (NaCAS) is a very useful ingredient in food industry because of its nutritional and functional properties. Acidification produces a gel structure as a result of the dissociation and aggregation of caseinic fractions. Formation of these protein gels can be made by the slow reduction of pH through the addition of glucono-delta-lactone (GDL). Depending on its concentration and temperature, hydrolysis speed of GDL can affect the grade of hardness and elasticity of the formed gel. This study evaluated the effect on the formation and structure of protein gels induced by different relations of GDL through analysis of digital images obtained in an inverted conventional microscope and a confocal microscope. The entropy, smoothness and variance decrease with the added GDL quantity, but the uniformity increases. Results confirm that the texture depends on gelification speed, which is directly related to the amount of added GDL. This digital image analysis technique using conventional or confocal microscopy is, therefore, suitable and very useful for the texture analysis of acid gels formed by different GDL/NaCAS rates.

References

  1. Braga, A., Menossi, M., Cunha, R. (2006). The Effect of the Glucono-[delta]-Lactone/Caseinate Ratio on Sodium Caseinate Gelation. International Dairy Journal, 16, (5), 389-398.
  2. de Kruif, C. G. (1997). Skim Milk Acidification. Journal of Colloid and Interface Science, 185, (1), 19-25.
  3. Gonzalez, R., Woods, E. (2002). Digital Imaging Processing. 2nd edition, Prentice Hall.
  4. Haralick R. (1979). Statistical and Structural Approach to Texture. Proceeding of IEEE, Vol. 67 (5).
  5. Jensen, J.R. (1996). Introductory Digital Image Processing, Prentice Hall.
  6. Lucey, J. (2002). Formation and Physical Properties of Milk Protein Gels, J. Dairy Sci. 85, 281-294.
  7. Mulvihill, D., Fox, P. (1989). Caseins and Manufactured. In Development in Dairy Chemistry, Ed., P. F. F., Ed. Elsevier Applied Science: London & New York,; (Vol. 4, pp. 97-130).
  8. Nespolo, C., Reggiardo, A., Mancilla Canales, M., Wagner, J., Alvarez, E., Brandelli, A, Risso, P. (2010). Effect of Calcium on Ovine Caseinate Functional Properties. Journal of Chemical and Engineering Data, in press.
  9. Ruis, H., Venema, P., van der Linden, E. (2007). Relation Between pH-Induced Stickiness and Gelation Behaviour of Sodium Caseinate Aggregates as Determined by Light Scattering and Rheology. Food Hydrocolloids, 21, (4), 545-554.
  10. Walstra, P. (1984). Dairy Chemistry and Physics. New York, USA; John Wiley & Sons; (pp. 1-225).
  11. Yan, L., Yue, W., Jiye, C. (2008). Atomic Force Microscopic Investigations on Pectin Gelling Made Under Optimum Conditions. Chemical Journal on Internet 10, (9), P. 45
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Paper Citation


in Harvard Style

Pablo Costa J., Castellini H., Risso P. and Riquelme B. (2011). TEXTURE ANALYSIS OF MILK PROTEIN GELS USING DIGITAL IMAGE ANALYSIS . In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011) ISBN 978-989-8425-36-2, pages 322-325. DOI: 10.5220/0003289303220325


in Bibtex Style

@conference{bioinformatics11,
author={Juan Pablo Costa and Horacio Castellini and Patricia Risso and Bibiana Riquelme},
title={TEXTURE ANALYSIS OF MILK PROTEIN GELS USING DIGITAL IMAGE ANALYSIS },
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)},
year={2011},
pages={322-325},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003289303220325},
isbn={978-989-8425-36-2},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)
TI - TEXTURE ANALYSIS OF MILK PROTEIN GELS USING DIGITAL IMAGE ANALYSIS
SN - 978-989-8425-36-2
AU - Pablo Costa J.
AU - Castellini H.
AU - Risso P.
AU - Riquelme B.
PY - 2011
SP - 322
EP - 325
DO - 10.5220/0003289303220325