MULTI-ANALYTE DETECTION FOR BIOLOGICAL FLUIDS - Towards Continous Monitoring of Glucose, Ionized Calcium and pH using a Viscometric Affinity Biosensor

Christophe Boss, Eric Meurville, Peter Ryser, Frédéric Schmitt, Lucienne Juillerat-Jeanneret, Pablo Dosil-Rosende, Desdemona De Souza

Abstract

We present a viscometric affinity biosensor that can potentially allow continuous multi-analyte monitoring in biological fluids like blood or plasma. The sensing principle is based on the detection of viscosity changes of a polymeric solution which has a selective affinity for the analyte of interest. The chemico-mechanical sensor incorporates an actuating piezoelectric diaphragm, a sensing piezoelectric diaphragm and a flow-resisting microchannel for viscosity detection. A free-standing Anodic Alumina Oxide (AAO) porous nano-membrane is used as selective interface. A glucose-sensitive sensor was fabricated and extensively assessed in buffer solution. The sensor reversibility, stability and sensitivity were excellent during at least 65 hours. Results showed also a good degree of stability for a long term measurement (25 days). The sensor behaviour was furthermore tested in fetal bovine serum (FBS). The obtained results for glucose sensing are very promising, indicating that the developed sensor is a candidate for continuous monitoring in biological fluids. Sensitive solutions for ionized calcium and pH are currently under development and should allow multi-analyte sensing in the near future.

References

  1. Ballerstadt, R., Polak, A., Beuhler, A., Frye, J., 2004. In Vitro long-term performance study of a near-infrared fluorescence affinity sensor for glucose monitoring. Biosensors & Bioelectronics. Elsevier.
  2. Boss, C., Meurville, E., Sallese, J.M., Ryser, P., 2009. Novel chemico-mechanical approach towards longterm implantable glucose sensing. Procedia Chemistry. Elsevier.
  3. Huang, X., Li, S., Schultz, J., Wang, Q., Lin, Q., 2009. A MEMS affinity glucose sensor using a biocompatible glucose-responsive polymer. Sensor and Actuators B: Chemical. Elsevier.
  4. Huang, X., Li, S., Schultz, J., Wang, Q., Lin, Q., 2009. A Capacitive MEMS Viscometric Sensor for Affinity Detection of Glucose. J Micromech Microeng. IEEE.
  5. Kuenzi, S., Meurville, E., Ryser, P., 2010. Automated characterization of dextran/concanavalin A mixtures - A study of sensitivity and temperature dependence at low viscosity as basis for an implantable glucose sensor. Sensor and Actuators B: Chemical. Elsevier.
  6. Tierney, S., Falch, B., Hjelme, D., Stokke, B., 2009. Determination of Glucose Levels Using a Functionalized Hydrogel-Optical Fiber Biosensor: Toward Continuous Monitoring of Blood Glucose in Vivo. Anal. Chem. ACS.
Download


Paper Citation


in Harvard Style

Boss C., Meurville E., Ryser P., Schmitt F., Juillerat-Jeanneret L., Dosil-Rosende P. and De Souza D. (2011). MULTI-ANALYTE DETECTION FOR BIOLOGICAL FLUIDS - Towards Continous Monitoring of Glucose, Ionized Calcium and pH using a Viscometric Affinity Biosensor . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 295-298. DOI: 10.5220/0003350902950298


in Bibtex Style

@conference{biodevices11,
author={Christophe Boss and Eric Meurville and Peter Ryser and Frédéric Schmitt and Lucienne Juillerat-Jeanneret and Pablo Dosil-Rosende and Desdemona De Souza},
title={MULTI-ANALYTE DETECTION FOR BIOLOGICAL FLUIDS - Towards Continous Monitoring of Glucose, Ionized Calcium and pH using a Viscometric Affinity Biosensor},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={295-298},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003350902950298},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - MULTI-ANALYTE DETECTION FOR BIOLOGICAL FLUIDS - Towards Continous Monitoring of Glucose, Ionized Calcium and pH using a Viscometric Affinity Biosensor
SN - 978-989-8425-37-9
AU - Boss C.
AU - Meurville E.
AU - Ryser P.
AU - Schmitt F.
AU - Juillerat-Jeanneret L.
AU - Dosil-Rosende P.
AU - De Souza D.
PY - 2011
SP - 295
EP - 298
DO - 10.5220/0003350902950298