LEAST-SQUARES ESTIMATION OF NANOPORE CHANNEL CONDUCTANCE IN VOLTAGE-VARYING EXPERIMENTS

Christopher R. O'Donnell, William B. Dunbar

Abstract

Step-changing and sinusoidal voltage patterns have expanded the capabilities of the nanopore instrument for single molecule manipulation and measurement. A challenge with voltage-varying experiments is that capacitance in the system is excited and masks the contribution of the nanopore channel conductance in the measured current. The conductance is the parameter that can be used to infer the dynamics of the complex (e.g., DNA, or DNA-protein) in the pore. We present a least-squares parameter estimation (LSPE) algorithm for estimating the channel conductance under voltage-varying conditions, including step and sinusoidal voltages, with the objective of inferring the channel conductance parameter as continuously as possible. The algorithm is shown to recover the conductance faster than by waiting for capacitive transients to settle in step-voltage experiments, and provides accurate continuous conductance estimates in sinusoidal voltage experiments, with realistic noise levels superimposed on the measurements.

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Paper Citation


in Harvard Style

O'Donnell C. and Dunbar W. (2012). LEAST-SQUARES ESTIMATION OF NANOPORE CHANNEL CONDUCTANCE IN VOLTAGE-VARYING EXPERIMENTS . In Proceedings of the International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2012) ISBN 978-989-8425-89-8, pages 270-275. DOI: 10.5220/0003790502700275


in Bibtex Style

@conference{biosignals12,
author={Christopher R. O'Donnell and William B. Dunbar},
title={LEAST-SQUARES ESTIMATION OF NANOPORE CHANNEL CONDUCTANCE IN VOLTAGE-VARYING EXPERIMENTS},
booktitle={Proceedings of the International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2012)},
year={2012},
pages={270-275},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003790502700275},
isbn={978-989-8425-89-8},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2012)
TI - LEAST-SQUARES ESTIMATION OF NANOPORE CHANNEL CONDUCTANCE IN VOLTAGE-VARYING EXPERIMENTS
SN - 978-989-8425-89-8
AU - O'Donnell C.
AU - Dunbar W.
PY - 2012
SP - 270
EP - 275
DO - 10.5220/0003790502700275