Hemorrhage Control by Short Electrical Pulses - In Vivo Experiments

Guy Malki, Ofer Barnea, Yossi Mandel

Abstract

An internal hemorrhagic shock is one of the leading causes of death in the battlefield and other trauma events. However the application of direct pressure, as in the treatment of an external hemorrhage, is not possible. Most common techniques to achieve vasoconstriction are through heat; yet heating causes irreversible destruction of organ tissues. Therefore, there is a need for a non-thermal based technology for hemorrhage control. The current research describes, for the first, an attempt to reduce the amount of bleeding in animal model liver injuries by using electrical pulses treatment (EPT). In the experiments, which were performed on 28 rats and 14 rabbits, a short (25µs and 50µs) EPT was applied to the treatment groups and the amount of bleeding was compared to the non-treatment (NT) groups. A reduction of 60%, 36% and 44% in blood volume, was found in the 25µs-rats, 50µs-rats and 25µs-rabbits EPT groups, respectively (P<0.001). Also, it was found that the hemorrhage control was not caused by the mechanical pressure applied by the electrodes, and there was no evidence for thermal coagulation. Further research is needed to fully expose the potential of this treatment and the modality for hemorrhage control in civilian and military settings.

References

  1. Burgess, S., Zderic, V., & Vaezy, S. (2007). Image-guided acoustic hemostasis for hemorrhage in the posterior liver. Ultrasound in medicine & biology, 33(1), 113-9.
  2. Gehl, J., Skovsgaard, T., & Mir, L. M. (2002). Vascular reactions to in vivo electroporation: characterization and consequences for drug and gene delivery. Biochimica et biophysica acta, 1569(1-3), 51-8.
  3. Graham, J., Bronskill, & Henkelman, (1998). Time and Temperature Dependence of MR Parameters.pdf. MR Parameters and Thermal Coagulation, 39, 198-203.
  4. Guarini, S. (1971). Model of Arterial Thrombosis in Rats, 8719(96).
  5. Hildreth, J., Wisner, D. H., Matsuoka, T. (1996). Uncontrolled Hemorrhage from Parenchymal Injury?: Is Resuscitation Helpful?? The Journal of Trauma: Injury, Infection, and Critical Care, 40(6), 915-922.
  6. Hladovec J. (1975). A quantitative model of venous stasis thrombosis in rats. Physiol Bohemoslov., 24(6), 551-4.
  7. Matsuoka, T., & Wisner, D. H., (1996). Resuscitation of uncontrolled liver hemorrhage: effects on bleeding, oxygen delivery, and oxygen consumption. The Journal of Trauma: Injury, Infection, and Critical Care, 41(3), 439-45.
  8. Matsuoka, Ishida, M. and Konishi, (2004). Numerical study of temperature distribution in tissue for thermalcoagulation therapy..pdf. Journal of Magnetism and Magnetic Materials, 272-276, 2426- 2427.
  9. Matsushima Y, Takahashi E, Hagiwara K, Konaka C, Miura H, Kato H, K. Y., (1994). Clinical and experimental studies of anti-tumoral effects of electrochemical therapy (ect) alone or in combinationwith chemotherapy. Eur J Surgery, 574(59-67).
  10. Palanker, D., Vankov, A., Freyvert, Y., & Huie, P., (2008). Pulsed electrical stimulation for control of vasculature: temporary vasoconstriction and permanent thrombosis. Bioelectromagnetics, 29(2), 100-7.
  11. Precup, C. G., Gonganau-Nitu, D., Scurtu, R. R., Dindelegan, G., Biro, A., Soritau, O., Crisan, C., et al., (2010). Assessement by laser Doppler of the peripheral tumour perfusion after radiofrequency ablation for colorectal liver mestasis--experimental study. Chirurgia (Bucharest, Romania?: 1990), 105(1), 71-6.
  12. Ramirez, Orlowski, Bindoula, Dzodic, Ardouin, Bognel, Jr, B., et al. (1998). Electrochemotherapy on liver tumours in rabbits. British Joumal of Cancer, 77(12), 2104-2111.
  13. Sersa, G., Jarm, T., Kotnik, T., Coer, a, Podkrajsek, M., Sentjurc, M., Miklavcic, D., et al., (2008, January 29). Vascular disrupting action of electroporation and electrochemotherapy with bleomycin in murine sarcoma. British journal of cancer.
  14. Sersa, G. Parkins, C. . ., & Chaplin, D. J., (1999). Tumour Blood Flow Changes Induced by Application of Electric Pulses. European Journal of Cancer, 35(4), 672-677.
  15. Vaezy, S., Martin, R., Schmiedl, U., Caps, M., Taylor, S., Beach, K., Carter, S., et al., (1997, January). Liver hemostasis using high-intensity focused ultrasound. Ultrasound in medicine & biology.
  16. Yu-ling Xin, Fu-zhou Xue, Bing-sheng Ge, Feng-rui Zhao, Bin Shi, and W. Z., (1997). Electrochemical Treatment of Lung Cancer. Bioelectromagnetics, 18, 8-13.
Download


Paper Citation


in Harvard Style

Malki G., Barnea O. and Mandel Y. (2013). Hemorrhage Control by Short Electrical Pulses - In Vivo Experiments . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013) ISBN 978-989-8565-34-1, pages 103-107. DOI: 10.5220/0004190001030107


in Bibtex Style

@conference{biodevices13,
author={Guy Malki and Ofer Barnea and Yossi Mandel},
title={Hemorrhage Control by Short Electrical Pulses - In Vivo Experiments},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)},
year={2013},
pages={103-107},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004190001030107},
isbn={978-989-8565-34-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)
TI - Hemorrhage Control by Short Electrical Pulses - In Vivo Experiments
SN - 978-989-8565-34-1
AU - Malki G.
AU - Barnea O.
AU - Mandel Y.
PY - 2013
SP - 103
EP - 107
DO - 10.5220/0004190001030107