Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale

Joachim Walter, Natalia Schiefermeier, Nina Hobi, Benjamin Grau, Christine E. Bandtlow, Lukas A. Huber, Rainer Uhl, Sebastian Munck

Abstract

The plasma membrane is the outer limit of the animal cell. As such, it is both a border separating inside from outside and a signaling platform for interactions with the surroundings. Among these interactions are extracellular matrix contacts and adhesion sites. The membrane and its contact sites together with the underlying cytoskeleton undergo constant remodeling, which leads to changes of the cell shape. In addition to spatial information micro-topographical maps provide, information about the z-dimension and describe the position of the plasma membrane with respect to the distance to a given substrate. Here we address how to measure height differences in the plasma membrane and how to create topographical maps of the plasma membrane with nanometer resolution. We address the currently used methodologies along with their advantages and drawbacks. Moreover, we delineate a label-free method to obtain topographic maps of the plasma membrane that are corrected for differences in the refractive index of the membrane utilizing an interferometric approach with multiple wavelengths and a normalization procedure to account for changes in the refractive index in the membrane.

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Paper Citation


in Harvard Style

Walter J., Schiefermeier N., Hobi N., Grau B., Bandtlow C., Huber L., Uhl R. and Munck S. (2014). Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale . In Proceedings of the International Conference on Bioimaging - Volume 1: BIOIMAGING, (BIOSTEC 2014) ISBN 978-989-758-014-7, pages 31-40. DOI: 10.5220/0004752100310040


in Bibtex Style

@conference{bioimaging14,
author={Joachim Walter and Natalia Schiefermeier and Nina Hobi and Benjamin Grau and Christine E. Bandtlow and Lukas A. Huber and Rainer Uhl and Sebastian Munck},
title={Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale},
booktitle={Proceedings of the International Conference on Bioimaging - Volume 1: BIOIMAGING, (BIOSTEC 2014)},
year={2014},
pages={31-40},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004752100310040},
isbn={978-989-758-014-7},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioimaging - Volume 1: BIOIMAGING, (BIOSTEC 2014)
TI - Mapping Membrane Topology Label Free and Corrected for Changes in the Refractive Index of the Membrane on a Nanometer Scale
SN - 978-989-758-014-7
AU - Walter J.
AU - Schiefermeier N.
AU - Hobi N.
AU - Grau B.
AU - Bandtlow C.
AU - Huber L.
AU - Uhl R.
AU - Munck S.
PY - 2014
SP - 31
EP - 40
DO - 10.5220/0004752100310040