Assessing Composition of Coronary Thrombus in STEMI Patients - A Multiscale Approach to Charaterize Samples Obtained by Catheter Aspiration

Francesco Tessarolo, Emiliana Bonomi, Federico Piccoli, Fabiola Morat, Marta Rigoni, Iole Caola, Mattia Barbareschi, Patrizio Caciagli, Simone Muraglia, Roberto Bonmassari, Giandomenico Nollo

Abstract

Percutaneous catheter thrombectomy allows collecting coronary thrombi from patients with ST-segment elevation myocardial infarction, preserving most of the sample characteristics available for both visual assessment and compositional analysis. This study aimed at identifying the association between thrombus macroscopic aspect and composition and, secondly, correlations between composition and ischemic time. Aspirated thrombi were grouped into “white”, “red” or “mixed” according to their macroscopic appearance. Platelets, RBCs and fibrin were quantified on thrombus surface by SEM and on thrombus sections by a modified Carstairs histochemical staining. Fifty-three samples from 51 patients were included. The median [inter-quartile range] ischemic time was 210 [190-265] min. Seven (13%) “white”, 19(36%) “mixed” and 27(51%) “red” thrombi were macroscopically identified. Median thrombus composition assessed by SEM was 23[11-41]%, 43[26-62]%, and 24[11-37]% for platelets, RBCs and fibrin respectively. Median histological analysis was 10[5-26]%, 45[31-64]% and 30[18-49]% for the same components. Significant difference in composition were found between “white” and “red” thrombi, showing respectively a higher amount of platelets (p=0.003) and fibrin (p=0.007). No significant correlations were found between composition and ischemic time suggesting that plaque instability and thrombus formation can occur before the symptom onset.

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Paper Citation


in Harvard Style

Tessarolo F., Bonomi E., Piccoli F., Morat F., Rigoni M., Caola I., Barbareschi M., Caciagli P., Muraglia S., Bonmassari R. and Nollo G. (2014). Assessing Composition of Coronary Thrombus in STEMI Patients - A Multiscale Approach to Charaterize Samples Obtained by Catheter Aspiration . In Proceedings of the 2nd International Congress on Cardiovascular Technologies - Volume 1: CARDIOTECHNIX, ISBN 978-989-758-055-0, pages 5-12. DOI: 10.5220/0005144600050012


in Bibtex Style

@conference{cardiotechnix14,
author={Francesco Tessarolo and Emiliana Bonomi and Federico Piccoli and Fabiola Morat and Marta Rigoni and Iole Caola and Mattia Barbareschi and Patrizio Caciagli and Simone Muraglia and Roberto Bonmassari and Giandomenico Nollo},
title={Assessing Composition of Coronary Thrombus in STEMI Patients - A Multiscale Approach to Charaterize Samples Obtained by Catheter Aspiration},
booktitle={Proceedings of the 2nd International Congress on Cardiovascular Technologies - Volume 1: CARDIOTECHNIX,},
year={2014},
pages={5-12},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005144600050012},
isbn={978-989-758-055-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 2nd International Congress on Cardiovascular Technologies - Volume 1: CARDIOTECHNIX,
TI - Assessing Composition of Coronary Thrombus in STEMI Patients - A Multiscale Approach to Charaterize Samples Obtained by Catheter Aspiration
SN - 978-989-758-055-0
AU - Tessarolo F.
AU - Bonomi E.
AU - Piccoli F.
AU - Morat F.
AU - Rigoni M.
AU - Caola I.
AU - Barbareschi M.
AU - Caciagli P.
AU - Muraglia S.
AU - Bonmassari R.
AU - Nollo G.
PY - 2014
SP - 5
EP - 12
DO - 10.5220/0005144600050012