Essential Proteins and Functional Modules in the Host-Pathogen Interactions from Innate to Adaptive Immunity - C. albicans-zebrafish Infection Model

Chia-Chou Wu, Bor-Sen Chen

Abstract

The host and the pathogen are indispensable in the infectious diseases. Besides studying the host defensive and pathogen invasive mechanisms individually, the cross-species interactions, i.e., the host-pathogen interactions, become a novel and intense research subjects of the infectious diseases. In this study, two host-pathogen interaction networks are constructed for innate and adaptive immunity based on the time course microarray data of C. albicans-zebrafish infection model. The interaction variations in the host, pathogen, and host-pathogen regions are evaluated by comparing the two constructed networks. Those proteins of larger interaction variations stand for more pivotal roles in the transition from innate to adaptive immunity. Moreover, in the host-pathogen region, four significantly enriched functional modules are identified. Meanwhile, the interaction variations of these four functional groups imply the corresponding strategy shifts of the host and pathogen from innate to adaptive immunity. In view of these results, this study gives a systematic explanation about the transition from innate and adaptive immunity from functional modules perspective. Thus, this study provides potential targets for developing efficient therapies of the infectious diseases.

References

  1. Arnold, R., Boonen, K., Sun, M. G. F., and Kim, P. M. (2012). Computational analysis of interactomes: Current and future perspectives for bioinformatics approaches to model the host-pathogen interaction space. Methods, 57(4):508-518.
  2. Chao, C. C., Hsu, P. C., Jen, C. F., Chen, I. H., Wang, C. H., Chan, H. C., Tsai, P. W., Tung, K. C., Wang, C. H., Lan, C. Y., and Chuang, Y. J. (2010). Zebrafish as a model host for Candida albicans infection. Infection and Immunity, 78(6):2512-2521.
  3. Chen, Y. Y., Chao, C. C., Liu, F. C., Hsu, P. C., Chen, H. F., Peng, S. C., Chuang, Y. J., Lan, C. Y., Hsieh, W. P., and Wong, D. S. H. (2013). Dynamic transcript profiling of Candida albicans infection in zebrafish: A pathogen-host interaction study. PloS One, 8(9).
  4. Flynn, N. E., Patyrak, M. E., Seely, J. B., and Wu, G. Y. (2010). Glycine oxidation and conversion into amino acids in Saccharomyces cerevisiae and Candida albicans. Amino Acids, 39(2):605-608.
  5. Gerold, G., Abu Ajaj, K., Bienert, M., Laws, H. J., Zychlinsky, A., and de Diego, J. L. (2008). A Toll-like receptor 2-integrin ß3 complex senses bacterial lipopeptides via vitronectin. Nature Immunology, 9(7):761-768.
  6. Kopp, A., Gross, P., Falk, W., Bala, M., Weigert, J., Buechler, C., Neumeier, M., Scholmerich, J., and Schaffler, A. (2009). Fatty acids as metabolic mediators in innate immunity. European Journal of Clinical Investigation, 39(10):924-933.
  7. Kuo, Z. Y., Chuang, Y. J., Chao, C. C., Liu, F. C., Lan, C. Y., and Chen, B. S. (2013). Identification of infection- and defense-related genes via a dynamic host-pathogen interaction network using a Candida albicans-zebrafish infection model. Journal of Innate Immunity, 5(2):137-152.
  8. Leroy, Q. and Raoult, D. (2010). Review of microarray studies for host-intracellular pathogen interactions. Journal of Microbiological Methods, 81(2):81-95.
  9. Lohberger, A., Coste, A. T., and Sanglard, D. (2014). Distinct roles of Candida albicans drug resistance transcription factors TAC1, MRR1, and UPC2 in virulence. Eukaryotic Cell, 13(1):127-142.
  10. Meijer, A. H. and Spaink, H. P. (2011). Host-pathogen interactions made transparent with the zebrafish model. Current Drug Targets, 12(7):1000-1017.
  11. Odds, F. C. (1979). Candida and candidosis. University Park Press, Baltimore.
  12. Romani, L. (2011). Immunity to fungal infections. Nature Reviews Immunology, 11(4):275-288.
  13. Schier, A. F. (2013). Genomics zebrafish earns its stripes. Nature, 496(7446):443-444.
  14. Schmidt, F. and Volker, U. (2011). Proteome analysis of host-pathogen interactions: Investigation of pathogen responses to the host cell environment. Proteomics, 11(15):3203-3211.
  15. Tierney, L., Kuchler, K., Rizzetto, L., and Cavalieri, D. (2012). Systems biology of host-fungus interactions: turning complexity into simplicity. Current Opinion in Microbiology, 15(4):440-446.
  16. Trede, N. S., Langenau, D. M., Traver, D., Look, A. T., and Zon, L. I. (2004). The use of zebrafish to understand immunity. Immunity, 20(4):367-379.
  17. Wang, Y. C., Lin, C., Chuang, M. T., Hsieh, W. P., Lan, C. Y., Chuang, Y. J., and Chen, B. S. (2013). Interspecies protein-protein interaction network construction for characterization of host-pathogen interactions: a Candida albicans-zebrafish interaction study. BMC Systems Biology, 7.
  18. Wang, Y. C., Tsai, I. C., Lin, C., Hsieh, W. P., Lan, C. Y., Chuang, Y. J., and Chen, B. S. (2014). Essential functional modules for pathogenic and defensive mechanisms in Candida albicans infections. Biomed Research International.
  19. Wight, T. N., Kang, I., and Merrilees, M. J. (2014). Versican and the control of inflammation. Matrix Biology, 35:152-161.
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Paper Citation


in Harvard Style

Wu C. and Chen B. (2015). Essential Proteins and Functional Modules in the Host-Pathogen Interactions from Innate to Adaptive Immunity - C. albicans-zebrafish Infection Model . In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2015) ISBN 978-989-758-070-3, pages 17-25. DOI: 10.5220/0005201700170025


in Bibtex Style

@conference{bioinformatics15,
author={Chia-Chou Wu and Bor-Sen Chen},
title={Essential Proteins and Functional Modules in the Host-Pathogen Interactions from Innate to Adaptive Immunity - C. albicans-zebrafish Infection Model},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2015)},
year={2015},
pages={17-25},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005201700170025},
isbn={978-989-758-070-3},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2015)
TI - Essential Proteins and Functional Modules in the Host-Pathogen Interactions from Innate to Adaptive Immunity - C. albicans-zebrafish Infection Model
SN - 978-989-758-070-3
AU - Wu C.
AU - Chen B.
PY - 2015
SP - 17
EP - 25
DO - 10.5220/0005201700170025