Discrimination of Different Foodborne Pathogens onto Carbohydrate Microarrays Using Surface Plasmon Resonance Imaging

Emilie Bulard, Aurélie Bouchet-Spinelli, Patricia Chaud, André Roget, Roberto Calemczuk, Sébastien Fort, Thierry Livache

Abstract

Food safety is a public health challenge. Devices allowing early, fast, label-free and in situ detection of bacteria are of great interest to prevent outbreaks. Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 are foodborne pathogens which were responsible of 60% of the hospitalizations in the USA in 2011. In this study, we conceived a carbohydrate microarray in order to detect and discriminate these three food pathogenic bacteria. In less than 10 hours, from an initial bacterial suspension of 100 bacteria per mL, Surface Plasmon Resonance imaging allowed the detection and the discrimination of these bacteria while they were growing and interacting specifically with the carbohydrate microarray. Moreover, this device is easily regenerable and can be re-used: it is probably a promising tool to early detect bacteria in food.

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Paper Citation


in Harvard Style

Bulard E., Bouchet-Spinelli A., Chaud P., Roget A., Calemczuk R., Fort S. and Livache T. (2015). Discrimination of Different Foodborne Pathogens onto Carbohydrate Microarrays Using Surface Plasmon Resonance Imaging . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015) ISBN 978-989-758-071-0, pages 121-126. DOI: 10.5220/0005254901210126


in Bibtex Style

@conference{biodevices15,
author={Emilie Bulard and Aurélie Bouchet-Spinelli and Patricia Chaud and André Roget and Roberto Calemczuk and Sébastien Fort and Thierry Livache},
title={Discrimination of Different Foodborne Pathogens onto Carbohydrate Microarrays Using Surface Plasmon Resonance Imaging},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)},
year={2015},
pages={121-126},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005254901210126},
isbn={978-989-758-071-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2015)
TI - Discrimination of Different Foodborne Pathogens onto Carbohydrate Microarrays Using Surface Plasmon Resonance Imaging
SN - 978-989-758-071-0
AU - Bulard E.
AU - Bouchet-Spinelli A.
AU - Chaud P.
AU - Roget A.
AU - Calemczuk R.
AU - Fort S.
AU - Livache T.
PY - 2015
SP - 121
EP - 126
DO - 10.5220/0005254901210126