Development of an Automated System for Ex Vivo Measuring the Neuro Muscular Junction Functionality

Simona Pisu, Emanuele Rizzuto, Antonio Musarò, Zaccaria Del Prete

Abstract

The loss of functional connection between muscle and nerve is a crucial biological mechanism involved in several neuromuscular diseases, as Amyotrophic Lateral Sclerosis (ALS). ALS is a neurodegenerative disease associated with motor neuron degeneration, muscle atrophy and paralysis. In this context, the aim of this work is to characterize the functionality of the communication between muscle and nerve in mouse models by the development of new automated experimental methodologies. We developed an ex vivo technique based on the comparison between muscle contractile response due to membrane stimulation and muscle contractile response due to nerve stimulation. Since this latter stimulation bypasses the neuronal signalling, any difference between the two contractile responses may be related to NMJ alterations. Once the system and the stimulation protocol were set we started investigating the SOD1(G93A) mouse, the most studied animal model of ALS. Preliminary results from the transgenic model are in accordance with the literature, showing muscle contraction defects and NMJ impairment.

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Paper Citation


in Harvard Style

Pisu S., Rizzuto E., Musarò A. and Del Prete Z. (2015). Development of an Automated System for Ex Vivo Measuring the Neuro Muscular Junction Functionality . In Doctoral Consortium - DCBIOSTEC, (BIOSTEC 2015) ISBN , pages 36-41


in Bibtex Style

@conference{dcbiostec15,
author={Simona Pisu and Emanuele Rizzuto and Antonio Musarò and Zaccaria Del Prete},
title={Development of an Automated System for Ex Vivo Measuring the Neuro Muscular Junction Functionality},
booktitle={Doctoral Consortium - DCBIOSTEC, (BIOSTEC 2015)},
year={2015},
pages={36-41},
publisher={SciTePress},
organization={INSTICC},
doi={},
isbn={},
}


in EndNote Style

TY - CONF
JO - Doctoral Consortium - DCBIOSTEC, (BIOSTEC 2015)
TI - Development of an Automated System for Ex Vivo Measuring the Neuro Muscular Junction Functionality
SN -
AU - Pisu S.
AU - Rizzuto E.
AU - Musarò A.
AU - Del Prete Z.
PY - 2015
SP - 36
EP - 41
DO -