Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization

V. Errico, R. Molinaro, C. Gargioli, F. Ferranti, M. Dinescu, S. Cannata, G. Saggio, S. Rufini, A. Desideri


The membrane-substrate interactions have a topological valence and represent a level of information exchange between the cell and the extra-cellular matrix and/or between cells. The interactions can vary with boundary conditions and can be altered by varying the chemical and/or physical properties of the substrate. The alteration can presumably result in differentiation or specialization of the cells, but this fundamental aspect must still be fully understood. In such a frame, we investigated the levels of transcriptional coactivators YAP/TAZ throughout C2C12 differentiation on standard two-dimensional substrates and on polyethylene glycol-fibrinogen three-dimensional microenvironment. In detail, we observed that the use of a three-dimensional matrix permits an earlier differentiation in muscular cells when compared to standard bidimensional substrates. On such a basis, we want to investigate the modulation of a more regular threedimensional pattern on cells proliferation response and we propose a matrix, generable with multiphoton absorption, with regular aligned channels in order to overcome the current limitation in muscle regeneration techniques, so a possible tool to improve the myofibers formation and alignment.


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Paper Citation

in Harvard Style

Errico V., Molinaro R., Gargioli C., Ferranti F., Dinescu M., Cannata S., Saggio G., Rufini S. and Desideri A. (2016). Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 241-246. DOI: 10.5220/0005790402410246

in Bibtex Style

author={V. Errico and R. Molinaro and C. Gargioli and F. Ferranti and M. Dinescu and S. Cannata and G. Saggio and S. Rufini and A. Desideri},
title={Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)},

in EndNote Style

JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)
TI - Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization
SN - 978-989-758-170-0
AU - Errico V.
AU - Molinaro R.
AU - Gargioli C.
AU - Ferranti F.
AU - Dinescu M.
AU - Cannata S.
AU - Saggio G.
AU - Rufini S.
AU - Desideri A.
PY - 2016
SP - 241
EP - 246
DO - 10.5220/0005790402410246