High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection

Giuseppe Quero, Marco Consales, Renato Severino, Patrizio Vaiano, Alessandra Boniello, Annamaria Sandomenico, Menotti Ruvo, Anna Borriello, Laura Diodato, Simona Zuppolini, Michele Giordano, Immacolata Cristina Nettore, Annamaria Colao, Paolo Emidio Macchia, Flavio Santorelli, Antonello Cutolo, Andrea Cusano


We report an innovative fiber optic biosensor based on Long Period Gratings (LPGs) working in reflection configuration for real time monitoring of human Thyroglobulin (Tg), a protein marker of differentiated thyroid cancer. A standard LPG is first transformed in a practical probe working in reflection mode, and then it is coated with a single layer of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity and to provide, at the same time, the desired interfacial properties for a stable anti-Tg antibody. The functionalized reflection-type LPG biosensor clearly demonstrates the effectiveness and sensitivity of the developed biosensing platform, allowing the real time and label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels.


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Paper Citation

in Harvard Style

Quero G., Consales M., Severino R., Vaiano P., Boniello A., Sandomenico A., Ruvo M., Borriello A., Diodato L., Zuppolini S., Giordano M., Nettore I., Colao A., Macchia P., Santorelli F., Cutolo A. and Cusano A. (2016). High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 5: SmartMedDev, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 561-569. DOI: 10.5220/0005846705610569

in Bibtex Style

author={Giuseppe Quero and Marco Consales and Renato Severino and Patrizio Vaiano and Alessandra Boniello and Annamaria Sandomenico and Menotti Ruvo and Anna Borriello and Laura Diodato and Simona Zuppolini and Michele Giordano and Immacolata Cristina Nettore and Annamaria Colao and Paolo Emidio Macchia and Flavio Santorelli and Antonello Cutolo and Andrea Cusano},
title={High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 5: SmartMedDev, (BIOSTEC 2016)},

in EndNote Style

JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 5: SmartMedDev, (BIOSTEC 2016)
TI - High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection
SN - 978-989-758-170-0
AU - Quero G.
AU - Consales M.
AU - Severino R.
AU - Vaiano P.
AU - Boniello A.
AU - Sandomenico A.
AU - Ruvo M.
AU - Borriello A.
AU - Diodato L.
AU - Zuppolini S.
AU - Giordano M.
AU - Nettore I.
AU - Colao A.
AU - Macchia P.
AU - Santorelli F.
AU - Cutolo A.
AU - Cusano A.
PY - 2016
SP - 561
EP - 569
DO - 10.5220/0005846705610569