High-Throughput Sequencing Technology and Its Applications in Human Disease

Shuyang Deng


The high-throughput sequencing technology (HTS), also known as next generation sequencing, refers to the technological advances in DNA sequencing instrumentation that enable the generation of hundreds of thousands to millions of sequence reads per run. The advances of high-throughput, low cost and short timeconsuming democratizes HTS and paves the way for the development of a large number of novel HTS applications in basic science as well as in translational research areas, such as clinical diagnostics, agrigenomics, and forensic science. In recent years, HTS has been widely applied in solving biological problems, especially in human diseases field. In this review, we provide an overview of the evolution of HTS and discuss three important sequencing strategies HTS adopted, Roche/454, Illumina, SOLiD. We also take the example of exome sequencing and ChIP to summarize the application of HTS in human diseases.


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Paper Citation

in Harvard Style

Deng S. (2016). High-Throughput Sequencing Technology and Its Applications in Human Disease . In ISME 2016 - Information Science and Management Engineering IV - Volume 1: ISME, ISBN 978-989-758-208-0, pages 318-324. DOI: 10.5220/0006449703180324

in Bibtex Style

author={Shuyang Deng},
title={High-Throughput Sequencing Technology and Its Applications in Human Disease},
booktitle={ISME 2016 - Information Science and Management Engineering IV - Volume 1: ISME,},

in EndNote Style

JO - ISME 2016 - Information Science and Management Engineering IV - Volume 1: ISME,
TI - High-Throughput Sequencing Technology and Its Applications in Human Disease
SN - 978-989-758-208-0
AU - Deng S.
PY - 2016
SP - 318
EP - 324
DO - 10.5220/0006449703180324