Cytotoxicity and Effectiveness of 100% Mangosteen Pericarp Extract in Cleaning the Smear Layer of Root Canal Dentine

Sri Lestari

Abstract

Root canal preparation is one of the root canal treatment stages. It results in a smear layer attaching to the canal wall and can be pushed into the periapical tissue, causing infection. Therefore, it needs irrigation to prevent it. Irrigation materials used must be able to clean the smear layer and not be toxic to the periapical tissues. Irrigation material commonly used is NaOCl 2.5%. It has unique characteristics e.g. odorous, irritating, and an unpleasant taste. Mangosteen pericarp extract is used as an alternative because it contains active substances, i.e. xanthones and is expected to improve the characteristics of the existing irrigation materials. Objective: To determine the toxicity of mangosteen pericarp extract toward BHK-21 fibroblast cell lines and its ability in cleaning smear layer root canal dentine. Methods: Extraction of mangosteen pericarp was performed by the maceration method with 96% ethanol into a viscous extract 100%. Mangosteen pericarp extract 100% and NaOCl 2.5% toxicity was tested using the MTT assay method. The level of toxicity can be seen from the percentage life of the cell and the results were read by an ELISA reader. The capability in cleaning of root canal dentine samples were 10 superior first premolars with conventional preparation. Five samples were explained with 100% Mangosteen pericarp extract 100% then irrigated with sterile aquadest, and five samples were explained with NaOCl 2.5% then irrigated with sterile aquadest. After being dried, the samples were then examined for the cleaning of the smear layer with a Scanning Electron Microscope (SEM) with 3500x magnification. Results and Conclusion: Mangosteen pericarp extract 100% has a lower toxicity than 2.5% NaOCl. Mangosteen pericarp extract not considered toxic at 100% concentration,92.21% of the cells were alive. Mangosteen pericarp extract has the same ability to clean the smear layer as 2.5% NaOCl.

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Paper Citation


in Harvard Style

Lestari S. (2017). Cytotoxicity and Effectiveness of 100% Mangosteen Pericarp Extract in Cleaning the Smear Layer of Root Canal Dentine.In Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry - Volume 1: TIMNAS7-JSMID4, ISBN 978-989-758-341-4, pages 176-181. DOI: 10.5220/0007294501760181


in Bibtex Style

@conference{timnas7-jsmid417,
author={Sri Lestari},
title={Cytotoxicity and Effectiveness of 100% Mangosteen Pericarp Extract in Cleaning the Smear Layer of Root Canal Dentine},
booktitle={Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry - Volume 1: TIMNAS7-JSMID4,},
year={2017},
pages={176-181},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007294501760181},
isbn={978-989-758-341-4},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry - Volume 1: TIMNAS7-JSMID4,
TI - Cytotoxicity and Effectiveness of 100% Mangosteen Pericarp Extract in Cleaning the Smear Layer of Root Canal Dentine
SN - 978-989-758-341-4
AU - Lestari S.
PY - 2017
SP - 176
EP - 181
DO - 10.5220/0007294501760181