Level and Strain Number of Streptococcus Mutans in Toddlers after The Initial Acquisition

Udijanto Tedjosasongko, Katsuyuki Kozai

Abstract

Introduction. Streptococcus mutans (S mutans) are known as major bacteria in human dental caries. Previous experiments reported that S mutans could transmit between persons. Purpose. The purposes of this study were to determine the level and number of strain type of S mutans after the initial acquisition in children. Methods. Samples were the plaque of 39 children in range of age 0-5 years old of a day nursery in Hiroshima City Japan which were taken with toothbrush every month for 30 months. The S mutans isolate were taken by inoculation using mitis salivarius agar and BHI broth respectively. Identification was done by mannitol, sorbitol fermentation test and insoluble glucan synthesis test. The strain types of S mutans were determined by comparing the chromosomal DNA fingerprint of S mutans using restriction endonuclease EcoRI and HaeIII. Results. Twenty-one children (54%) acquired S mutans and 18 children (46%) remain free. The level of S mutans were increased gradually and changed irregularly; rapidly increased and remained at high level; and remained at low level. Seventeen children acquired 1 strain type, 3 children acquired 2 strain types, and 1 child acquired 3 strain types of S mutans from different sources. Conclusion. ThE study showed that the children could possessed more than one strain type of S mutans from different sources and the level of S mutans after initial acquisition period changed variously.

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Paper Citation


in Harvard Style

Tedjosasongko U. and Kozai K. (2017). Level and Strain Number of Streptococcus Mutans in Toddlers after The Initial Acquisition.In Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry - Volume 1: TIMNAS7-JSMID4, ISBN 978-989-758-341-4, pages 234-238. DOI: 10.5220/0007295602340238


in Bibtex Style

@conference{timnas7-jsmid417,
author={Udijanto Tedjosasongko and Katsuyuki Kozai},
title={Level and Strain Number of Streptococcus Mutans in Toddlers after The Initial Acquisition},
booktitle={Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry - Volume 1: TIMNAS7-JSMID4,},
year={2017},
pages={234-238},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007295602340238},
isbn={978-989-758-341-4},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry - Volume 1: TIMNAS7-JSMID4,
TI - Level and Strain Number of Streptococcus Mutans in Toddlers after The Initial Acquisition
SN - 978-989-758-341-4
AU - Tedjosasongko U.
AU - Kozai K.
PY - 2017
SP - 234
EP - 238
DO - 10.5220/0007295602340238