Role of Allogeneic NK Cells Treatment in the Early Phase of Apoptosis on Poorly Differentiated Retinoblastoma Cells Culture

Hendrian Dwikoloso Soebagjo, Fitria Kusumastuti, Parma Ratna Jaya, Susy Fatmariyanti, Endang Retnowati, Ugroseno S Yudho Bintoro

2017

Abstract

Introduction: Retinoblastoma is a retinal malignant tumor because of mutations on chromosome 13q14. This is associated with apoptosis deregulation processes. Both autologous or allogeneic NK cells play their roles in both innate and adaptive immune systems. They can stimulate apoptosis through several mechanisms. Methods: This study determined the role of allogeneic NK cells in retinoblastoma cell apoptosis. Poorly differentiated retinoblastoma tissues were tested with SDS-PAGE. Allogeneic NK cells were isolated from peripheral blood of the healthy family members of patients. There were two culture groups consisting of retinoblastoma cells only as the control and retinoblastoma treated with allogeneic NK cells as the treated group. Each group consisted of 10 well plates. Examinations of the expression of Bcl-2, Caspase-3, and Apoptosis were performed by the flowcytometry method. Result: The SDS PAGE electrophoresis tests showed strong expression of proteins with molecular weights of 14, 19, 28, 35, 53 and 85 kDa. The percentage of early-phase apoptosis is higher than late phase. There were significant correlations (α <0.05) between Bcl-2, Caspase-3, and the ratio of both on cell apoptosis. Conclusion: In conclusion, allogeneic NK cells play a role in retinoblastoma cell apoptosis, especially in the early phase. It is expected to be one of the new strategies in cancer immunotherapy

Download


Paper Citation


in Harvard Style

Soebagjo H., Kusumastuti F., Jaya P., Fatmariyanti S., Retnowati E. and Bintoro U. (2017). Role of Allogeneic NK Cells Treatment in the Early Phase of Apoptosis on Poorly Differentiated Retinoblastoma Cells Culture.In Proceedings of the International Meeting on Regenerative Medicine - Volume 1: IMRM, ISBN 978-989-758-334-6, pages 194-201. DOI: 10.5220/0007318701940201


in Bibtex Style

@conference{imrm17,
author={Hendrian Dwikoloso Soebagjo and Fitria Kusumastuti and Parma Ratna Jaya and Susy Fatmariyanti and Endang Retnowati and Ugroseno S Yudho Bintoro},
title={Role of Allogeneic NK Cells Treatment in the Early Phase of Apoptosis on Poorly Differentiated Retinoblastoma Cells Culture},
booktitle={Proceedings of the International Meeting on Regenerative Medicine - Volume 1: IMRM,},
year={2017},
pages={194-201},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007318701940201},
isbn={978-989-758-334-6},
}


in EndNote Style

TY - CONF

JO - Proceedings of the International Meeting on Regenerative Medicine - Volume 1: IMRM,
TI - Role of Allogeneic NK Cells Treatment in the Early Phase of Apoptosis on Poorly Differentiated Retinoblastoma Cells Culture
SN - 978-989-758-334-6
AU - Soebagjo H.
AU - Kusumastuti F.
AU - Jaya P.
AU - Fatmariyanti S.
AU - Retnowati E.
AU - Bintoro U.
PY - 2017
SP - 194
EP - 201
DO - 10.5220/0007318701940201