Anopheles Vagus Larval Midgut Damage as an Effect of Areca Catechu L. Seed Extract

Majematang Mading, Yeni Puji Lestari, Etik Ainun Rohmah, Budi Utomo, Heny Arwati, Subagyo Yotopranoto

2017

Abstract

Abstract: The Anopheles mosquito is the main transmitter of malaria and filariasis. The use of bioinsecticide is an alternative way to reduce the negative impacts of synthetic insecticides. Areca catechu L. seeds contain secondary metabolites that have bioinsectisidal effects. The aim of this study was to analyze the effects of A. catechu L. seed extract on the An. vagus larvae midgut. This study was a laboratory experimental research designed as a post test only control group design. A. catechu L. seed from Southwest Sumba Regency was extracted using ultrasonic methods. Larvae used in this study were Anopheles vagus instar III or early IV that divided into seven groups of the extract concentration (500; 1250; 2000; 2750; 3500; 4250 and 5000 ppm) and a control group to ascertain the toxicity of the extract. Each group consists of twenty five larvae. The histopathology of the larval midgut was stained with Hematoxyllin-Eosin and observed using light microscopy 24 hours after exposure. An histopathological examination of the larval midgut showed the mild, moderate and severe damage to the larval midgut after 24 hours exposure. Areca catechu L. seed extract can lead to changes involving damage to the larval midgut. Areca catechu L. seed extract can be recommended to be used effectively as a natural larvicidal mosquito control program. Further research is needed to determine the active ingredient.

Paper Citation

in Harvard Style

Mading M., Puji Lestari Y., Ainun Rohmah E., Utomo B., Arwati H. and Yotopranoto S. (2017). Anopheles Vagus Larval Midgut Damage as an Effect of Areca Catechu L. Seed Extract.In Proceedings of Surabaya International Physiology Seminar - Volume 1: SIPS, ISBN 978-989-758-340-7, pages 192-197. DOI: 10.5220/0007335501920197

in Bibtex Style

@conference{sips17,
author={Majematang Mading and Yeni Puji Lestari and Etik Ainun Rohmah and Budi Utomo and Heny Arwati and Subagyo Yotopranoto},
title={Anopheles Vagus Larval Midgut Damage as an Effect of Areca Catechu L. Seed Extract},
booktitle={Proceedings of Surabaya International Physiology Seminar - Volume 1: SIPS,},
year={2017},
pages={192-197},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007335501920197},
isbn={978-989-758-340-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of Surabaya International Physiology Seminar - Volume 1: SIPS,
TI - Anopheles Vagus Larval Midgut Damage as an Effect of Areca Catechu L. Seed Extract
SN - 978-989-758-340-7
AU - Mading M.
AU - Puji Lestari Y.
AU - Ainun Rohmah E.
AU - Utomo B.
AU - Arwati H.
AU - Yotopranoto S.
PY - 2017
SP - 192
EP - 197
DO - 10.5220/0007335501920197