Isolation and Characterization of a Petroleum-Degrading Pseudoalteromonas Haloplanktis Strain from the Digestive Tract of Perinereis Aibuhitensis (Polychaete)

Bin Wang, Baidong Zhang, Yibing Zhou

Abstract

The application of bioremediation approaches employing hydrocarbon-utilizing microorganisms to remove petroleum hydrocarbons from oil spills is an area of research that has gained extensive attention and has been widely investigated. In the present study, attempts have been made to isolate and characterize hydrocarbon-utilizing microorganisms immobilized in the digestive tract of Perinereis aibuhitensis. Isolate SC11-3, a potent petroleum-degrading organism, from Perinereis aibuhitensis gut samples was identified as Pseudoalteromonas sp. A detailed morphological, biochemical, and 16S rDNA sequence analysis revealed that it was closely related to Pseudoalteromonas haloplanktis. The isolate SC11-3 was capable of consuming about 40% diesel within 15 days from the medium containing 1 ml L-1 of oil. Furthermore, it was observed that the degrading efficiency of the isolate SC11-3 was significantly enhanced up to approximately 90% when the medium was supplemented with 4 g L-1 of glucose, indicating the possible occurrence of co-metabolism during the process of petroleum degradation by the bacterium. Our study reported an isolate of petroleum-degrading bacterium and its potential co-metabolism mechanism in oil degradation processes, which will provide new insight into in situ bioremediation of multi-biological systems.

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in Harvard Style

Wang B., Zhang B. and Zhou Y. (2018). Isolation and Characterization of a Petroleum-Degrading Pseudoalteromonas Haloplanktis Strain from the Digestive Tract of Perinereis Aibuhitensis (Polychaete).In Proceedings of the International Workshop on Environment and Geoscience - Volume 1: IWEG, ISBN 978-989-758-342-1, pages 24-31. DOI: 10.5220/0007425200240031


in Bibtex Style

@conference{iweg18,
author={Bin Wang and Baidong Zhang and Yibing Zhou},
title={Isolation and Characterization of a Petroleum-Degrading Pseudoalteromonas Haloplanktis Strain from the Digestive Tract of Perinereis Aibuhitensis (Polychaete)},
booktitle={Proceedings of the International Workshop on Environment and Geoscience - Volume 1: IWEG,},
year={2018},
pages={24-31},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007425200240031},
isbn={978-989-758-342-1},
}


in EndNote Style

TY - CONF

JO - Proceedings of the International Workshop on Environment and Geoscience - Volume 1: IWEG,
TI - Isolation and Characterization of a Petroleum-Degrading Pseudoalteromonas Haloplanktis Strain from the Digestive Tract of Perinereis Aibuhitensis (Polychaete)
SN - 978-989-758-342-1
AU - Wang B.
AU - Zhang B.
AU - Zhou Y.
PY - 2018
SP - 24
EP - 31
DO - 10.5220/0007425200240031