Osteogenic Responses of Murine Pre-osteoblastic Cells to Anodized TiO2 Surfaces

X. H. Piao, E. R. Na, J. W. Moon, Y. J. Kim

Abstract

Anodic oxidation of titanium surfaces by electrochemical method is one of methods for enhancement of osseointegration. This study was to evaluate the surface characteristics and cell response of titanium samples modified by different treatment conditions. The samples were divided into 4 groups. Group I was anodized TiO2 film using a constant voltage, 270 V for 30 seconds. Group II was anodized TiO2 film using a constant voltage, 270 V for 60 seconds. Group III was anodized TiO2 film using a constant voltage, 270 V for 90 seconds. The results were as follows; XRD analysis showed that the crystallinity of anodic oxide film was composed of anatase and rutile. With increasing time for anodization, the intensity of the TiO2 peaks for anatase phase decreased, while TiO2 peak for rutile phase increased. In MTT assay, there was no significant difference in the response of fetal rat calvarial cells to anodized titanium surfaces with different treatment conditions. The group II and III showed higher ALP activity levels compared with control and group I (p<0.01). These results suggest that anodized TiO2 surfaces treated at 60 and 90 seconds should promote cellular activity of osteoblasts compared with machined Ti surface.

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Paper Citation


in Harvard Style

Piao X., Na E., Moon J. and Kim Y. (2018). Osteogenic Responses of Murine Pre-osteoblastic Cells to Anodized TiO2 Surfaces.In Proceedings of the International Workshop on Materials, Chemistry and Engineering - Volume 1: IWMCE, ISBN 978-989-758-346-9, pages 442-449. DOI: 10.5220/0007439904420449


in Bibtex Style

@conference{iwmce18,
author={X. H. Piao and E. R. Na and J. W. Moon and Y. J. Kim},
title={Osteogenic Responses of Murine Pre-osteoblastic Cells to Anodized TiO2 Surfaces},
booktitle={Proceedings of the International Workshop on Materials, Chemistry and Engineering - Volume 1: IWMCE,},
year={2018},
pages={442-449},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007439904420449},
isbn={978-989-758-346-9},
}


in EndNote Style

TY - CONF

JO - Proceedings of the International Workshop on Materials, Chemistry and Engineering - Volume 1: IWMCE,
TI - Osteogenic Responses of Murine Pre-osteoblastic Cells to Anodized TiO2 Surfaces
SN - 978-989-758-346-9
AU - Piao X.
AU - Na E.
AU - Moon J.
AU - Kim Y.
PY - 2018
SP - 442
EP - 449
DO - 10.5220/0007439904420449