Differences of Caspase-3 Expression in Liver and Spleen of Rattus norvegicus Infected with Streptococcus pyogenes and Acinetobacter baumannii

Nurul Amalia, Agung Dwi Wahyu Widodo, Willy Sandhika

Abstract

Streptococcus pyogenes as exotoxin-producing bacteria are the most common cause of pharyngitis infection. Acinetobacter baumannii as endotoxin-producing bacteria are found in nosocomial infections. The infections that has already spread to all organs with decreased immune system undergo apoptosis in liver and spleen. Caspase-3 is a death protease most commonly activated by apoptotic mediator. The higher the caspase-3 expression, the higher the severity of the disease, which can cause the organ to experience dysfunction or failure. This study was aimed to observe the expression of caspase-3 in the liver and spleen of Rattus norvegicus, infected by Streptococcus pyogenes and Acinetobacter baumannii. This is a true experimental with a post-test only control-group design. The healthy rats were randomly selected and injected with 1ml (PZ, suspension of bacteri A. baumannii and S. pyogenes) in the peritoneum in quadrant 3 for each group of experimental animals and observed for 24 hours. After 24 hours, surgery took place for the removal of the liver and spleen. Organ tissues where fixed into a formalin buffer and tissue was prepared for IHC caspase-3.The results showed that the mortality rate of Rattus norvegicus infected by A. baumannii was higher than those infected by S. pyogenes. Caspase-3 expression in the liver of A. baumannii group was 48, S. pyogenes was 22.5, and the control group was 9.5. The mean value of the caspase-3 index in the spleen of the A. baumannii group was 28.5, S. pyogenes was 17, and the control group was 4.

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in Harvard Style

Amalia N., Dwi Wahyu Widodo A. and Sandhika W. (2018). Differences of Caspase-3 Expression in Liver and Spleen of Rattus norvegicus Infected with Streptococcus pyogenes and Acinetobacter baumannii.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 258-262. DOI: 10.5220/0007541002580262


in Bibtex Style

@conference{icps18,
author={Nurul Amalia and Agung Dwi Wahyu Widodo and Willy Sandhika},
title={Differences of Caspase-3 Expression in Liver and Spleen of Rattus norvegicus Infected with Streptococcus pyogenes and Acinetobacter baumannii},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={258-262},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007541002580262},
isbn={978-989-758-348-3},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Differences of Caspase-3 Expression in Liver and Spleen of Rattus norvegicus Infected with Streptococcus pyogenes and Acinetobacter baumannii
SN - 978-989-758-348-3
AU - Amalia N.
AU - Dwi Wahyu Widodo A.
AU - Sandhika W.
PY - 2018
SP - 258
EP - 262
DO - 10.5220/0007541002580262