Cytokine Profile Analysis of IL - 1ß and TNF-a in Diabetic Patients Infected by C.albicans

Regina Purnama Dewi Iskandar, Retno Pudji Rahayu

Abstract

Background: The prevalence of Diabetes Mellitus (DM) in Indonesia in 2020 is expected to affect more than 7 million people. The disease would lead to complication and oral manifestations. One of the oral manifestations of DM is an oral infection caused by Candida albicans. The patients are often in an immunocompromised state with an aberrant immune response. It was reported that diabetic patients have a higher level of pro-inflammatory cytokines. Objectives: This study aims to analyze the cytokine profile of diabetic patients infected with C.albicans. Methods: The subjects consist of 33 people with oral candidiasis, who were grouped into regulated DM, unregulated DM, and non-DM individuals. HbA1C was measured to classify the patients into the regulated DM or unregulated DM groups. Diagnosis of oral candidiasis was based on the clinical appearance of oral mucosa and Papanicolaou staining. There was 7cc of peripheral blood that was obtained from all subjects to analyze IL-1β and TNF-α levels, using an indirect ELISA technique. The data were statistically analyzed using one-way ANOVA. Results: The level of IL-1β and TNF-α were significantly different (α = 0.05) between each group. On the contrary, there was no significant difference in IL-1β and TNF-α level between the regulated DM group and the control group. Conclusion: The oral candidosis plays a role in altering pro-inflammatory cytokines levels in individuals with DM, in both regulated and unregulated groups.

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Paper Citation


in Harvard Style

Purnama Dewi Iskandar R. and Pudji Rahayu R. (2018). Cytokine Profile Analysis of IL - 1ß and TNF-a in Diabetic Patients Infected by C.albicans.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 281-284. DOI: 10.5220/0007541402810284


in Bibtex Style

@conference{icps18,
author={Regina Purnama Dewi Iskandar and Retno Pudji Rahayu},
title={Cytokine Profile Analysis of IL - 1ß and TNF-a in Diabetic Patients Infected by C.albicans},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={281-284},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007541402810284},
isbn={978-989-758-348-3},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Cytokine Profile Analysis of IL - 1ß and TNF-a in Diabetic Patients Infected by C.albicans
SN - 978-989-758-348-3
AU - Purnama Dewi Iskandar R.
AU - Pudji Rahayu R.
PY - 2018
SP - 281
EP - 284
DO - 10.5220/0007541402810284