The Effect Collagen to Granuloma Structure annd Immune Response on Granuloma Tuberculosis Invitro Models

Ira Pengesti, Agung Dwi Wahyu Widodo, Jusak Nugraha

2018

Abstract

Mycobacterium tuberculosis is a bacterium causes pulmonary tuberculosis, acid-resistant bacteria, intracellular life and cause of death than other infectious diseases. The principle of immune response by the formation of granulomas that prevent bacteria spread to other cells. TNF-α plays an important role in the body's defense against intracellular bacterial infections and the invitro model maintaining granulomas and the role of collagen in maintaining granulomas that prevent infection from becoming active. The purpose of this study is to analyze the number of lymphocyte, monocytes/macrophages and levels of TNF-α between collagen and without collagen. This study used healthy PBMC cells in mycobacterium tuberculosis bacterial infection with MOI 1: 0.1 then divided into 2 groups the addition collagen and without collagen observation in day 1, day 3, day 5, and day 7. HE stained was performed to calculate lymphocyte, monocyte/ macrophage and supernatant cells used to check TNF-α levels by ELISA method. The results showed no effect of the addition of collagen to the number of lymphocytes, monocytes/macrophages and TNF-α levels. Levels of TNF-α highest on day 3 and decresed on day 7.

Paper Citation

in Harvard Style

Pengesti I., Widodo A. and Nugraha J. (2018). The Effect Collagen to Granuloma Structure annd Immune Response on Granuloma Tuberculosis Invitro Models.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 356-360. DOI: 10.5220/0007542803560360

in Bibtex Style

@conference{icps18,
author={Ira Pengesti and Agung Dwi Wahyu Widodo and Jusak Nugraha},
title={The Effect Collagen to Granuloma Structure annd Immune Response on Granuloma Tuberculosis Invitro Models},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={356-360},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007542803560360},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Effect Collagen to Granuloma Structure annd Immune Response on Granuloma Tuberculosis Invitro Models
SN - 978-989-758-348-3
AU - Pengesti I.
AU - Widodo A.
AU - Nugraha J.
PY - 2018
SP - 356
EP - 360
DO - 10.5220/0007542803560360