Isolation and Identification of Acanthus ilicifolius-Associated Fungi and Investigation of Their Antibacterial Activity

Farras Daffa Imtiyaz, Ferisa Lestari Nugrahayu, Kawidian Putri Bayu Alam, Insan Shiddiq, Hermin Pancasakti Kusuma Sari

Abstract

Antibacterial resistance is one of the biggest threats to the global health security today. Therefore, there is an urgency to find alternative treatment to combat the bacteria. This paper aimed to identify and assess the biological activity of Acanthus ilicifolius endophytic fungi, a medicinal plant originated from Asia used in ancient medicine. In this experiment, two strains of bacterial were used, namely Staphylococcus aureus and Pseudomonas aeruginosa. The tree samples were obtained from Jepara, Indonesia. Surface sterilization and purification were applied to isolate the fungal. Moreover, their antibacterial ability was tested using agar plug diffusion method. The study found that three isolates were broadly active against two types of pathogenic bacteria; one of them was AB1-isolate. In the identification of the colony, AB1-fungal have both black stain in the basal part and the outside layer, with rough structure and planted strongly to the basal medium. The other fungal isolates, namely AD1 and AB2, showed a bacteriostatic effect against bacterial test as well. Our results suggested that AB1-isolate, obtained from the stems, shows a good prospect to be a pharmaceutical candidate to inhibit bacterial growth that normally present in the infection cases.

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Paper Citation


in Harvard Style

Imtiyaz F., Nugrahayu F., Alam K., Shiddiq I. and Sari H. (2018). Isolation and Identification of Acanthus ilicifolius-Associated Fungi and Investigation of Their Antibacterial Activity.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 484-488. DOI: 10.5220/0007545504840488


in Bibtex Style

@conference{icps18,
author={Farras Daffa Imtiyaz and Ferisa Lestari Nugrahayu and Kawidian Putri Bayu Alam and Insan Shiddiq and Hermin Pancasakti Kusuma Sari},
title={Isolation and Identification of Acanthus ilicifolius-Associated Fungi and Investigation of Their Antibacterial Activity},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={484-488},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007545504840488},
isbn={978-989-758-348-3},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Isolation and Identification of Acanthus ilicifolius-Associated Fungi and Investigation of Their Antibacterial Activity
SN - 978-989-758-348-3
AU - Imtiyaz F.
AU - Nugrahayu F.
AU - Alam K.
AU - Shiddiq I.
AU - Sari H.
PY - 2018
SP - 484
EP - 488
DO - 10.5220/0007545504840488