Earphone Swab-Derived 126bp and 143bp mtDNA at D-loop Region - Electrophoretic Band Reaction on Room Temperature

Ahmad Yudianto, Simon Martin Manyanza Nzilibili

Abstract

Exploration of crime scene devices, leftover, and its surrounding is of significance in forensic identification due to its potential harbor of biological evidences. Such harboring of evidences proposes traceability under Locard’s principle. By body use-contact, devices such as earphones potentiate revelation of the unknown by analysis of cerumen or traces logged from ear. Despite of this usability, environmental challenge is suggested to impact the forensic vitality of the logged evidences. To understand the phenomena, this study applied 30 used earphones at 0, 1, 7, 14 and 20 days room temperature exposure. From the study; amplified electrophoretic reaction showed that, 143bp (HVS I, nt 16268-16410) mtDNA D-loop region reacted with a positive (+) detection only for day 1 (4 [66.67%] samples) and day 7 (3 [50%] samples) of room temperature exposure. Meanwhile, visualization of the results of 126bp (HVS II, nt 34-159) mtDNA D-loop region showed a positive (+) detection only for day 1 (2 [33.37%] of samples) and day 7 (6 [100%] of samples) of room temperature exposure. Data interpretation conclude the effect of room temperature on the trend and quality of earphone swab-derived DNA from day 1 to day 20.

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Paper Citation


in Harvard Style

Yudianto A. and Nzilibili S. (2018). Earphone Swab-Derived 126bp and 143bp mtDNA at D-loop Region - Electrophoretic Band Reaction on Room Temperature.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 499-503. DOI: 10.5220/0007545804990503


in Bibtex Style

@conference{icps18,
author={Ahmad Yudianto and Simon Martin Manyanza Nzilibili},
title={Earphone Swab-Derived 126bp and 143bp mtDNA at D-loop Region - Electrophoretic Band Reaction on Room Temperature},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={499-503},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007545804990503},
isbn={978-989-758-348-3},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Earphone Swab-Derived 126bp and 143bp mtDNA at D-loop Region - Electrophoretic Band Reaction on Room Temperature
SN - 978-989-758-348-3
AU - Yudianto A.
AU - Nzilibili S.
PY - 2018
SP - 499
EP - 503
DO - 10.5220/0007545804990503