The Effect of Tadoples (Rana catesbeiana) Serum on Total and Differential Leukocyte in Rats (Rattus norvegicus) That Have Been Induced With Dimetylbenz-α-anthracene as Animal MODEL of Skin Cancer

Nur Prabowo Dwi Cahyo, Prima Ayu Wibawati, Hardayay Primarizky, Ragil Angga Prastiya, Muhammad Thohawi Elziyad Purnama, Ratna Damayanti

Abstract

This study aimed to determine the effect of tadpoles (Rana catesbeiana) serum on total and differential leukocyte in animal model of white rat (Rattus norvegicus) that has been induced with skin cancer by Dimethylbenz-α-anthracene (DMBA). Male rats were induced by DMBA 20 mg/rat twice a week for 18 days to induce skin cancer. Tadpole’s serum was injected intracutaneously after cancer had been known. Negative control (K-) was not induced with DMBA and tadpole’s serum, while positive control grup (K+) was induced to DMBA. The treatment groups P1, P2, and P3 were induced with DMBA and injected tadpole’s serum 100%; 75%; 25%/rat/day. This study used a Completely Randomized Design (CRD). Data were analyzed with ANOVA and continued by Duncan multiple test. The results obtained average number ± SD of total leukocyte K-, K+, P1, P2, and P3 were 12000.00±3814.88,3975.00±2451.36,8650.00± 5470.83, 6390.50 ± 3007.18 and 5590.00 ± 1292.18 respectively. There are significant differences regarding an increase in number of total leukocyte on treatment, but there is not real difference between K+, P1, P2 and P3. The results shsowed average number of limphocyte and monocyte are not significant but it is signiffant in granulocyte. Based on the results, it can be concluded that tadpoles serum is effective to increase number of total leukocyte and differential leukocyte (especially in granulocyte) in animal model of rats induced skin cancer.

Download


Paper Citation


in Harvard Style

Cahyo N., Wibawati P., Primarizky H., Prastiya R., Purnama M. and Damayanti R. (2018). The Effect of Tadoples (Rana catesbeiana) Serum on Total and Differential Leukocyte in Rats (Rattus norvegicus) That Have Been Induced With Dimetylbenz-α-anthracene as Animal MODEL of Skin Cancer.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 556-560. DOI: 10.5220/0007546905560560


in Bibtex Style

@conference{icps18,
author={Nur Prabowo Dwi Cahyo and Prima Ayu Wibawati and Hardayay Primarizky and Ragil Angga Prastiya and Muhammad Thohawi Elziyad Purnama and Ratna Damayanti},
title={The Effect of Tadoples (Rana catesbeiana) Serum on Total and Differential Leukocyte in Rats (Rattus norvegicus) That Have Been Induced With Dimetylbenz-α-anthracene as Animal MODEL of Skin Cancer},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={556-560},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007546905560560},
isbn={978-989-758-348-3},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Effect of Tadoples (Rana catesbeiana) Serum on Total and Differential Leukocyte in Rats (Rattus norvegicus) That Have Been Induced With Dimetylbenz-α-anthracene as Animal MODEL of Skin Cancer
SN - 978-989-758-348-3
AU - Cahyo N.
AU - Wibawati P.
AU - Primarizky H.
AU - Prastiya R.
AU - Purnama M.
AU - Damayanti R.
PY - 2018
SP - 556
EP - 560
DO - 10.5220/0007546905560560