Optimization of Culture Conditions for Chlorella Vulgaris Using Corresponding Normal Surface and Determination of Coefficients for Batch Kinetics of Nitrogen Removal Rate

Y. Zou, X. C. Xu, X. J. Wang, F. L. Yang, S. S. Zhang

Abstract

This study investigated the effects of CO2, light intensity, and phosphorus concentration on rate of NH4-N removal by Chlorella vulgaris and determined kinetic coefficients, such as biokinetic coefficients (k) and reaction rate constant (Km), using Michaelis–Menten rate expression under optimal conditions. By using Box–Behnken design under conditions of light intensity at 7446 lx, 2% CO2 content, and phosphorus concentration at 40 mg/L, the model predicted 38.56% maximum NH4-N removal rate. Maximum NH4-N removal rate of 18.7% at light intensity of 11216 lx and CO2 content of 4.4% was further tested by using central composite rotatable design. Batch experimental results confirmed kinetic equations of removal rate by C. vulgarisat optimal conditions and determined kinetic coefficients of k = 11.49 d−1, and Km =64.49mg/L.

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Paper Citation


in Harvard Style

Zou Y., Xu X., Wang X., Yang F. and Zhang S. (2018). Optimization of Culture Conditions for Chlorella Vulgaris Using Corresponding Normal Surface and Determination of Coefficients for Batch Kinetics of Nitrogen Removal Rate.In Proceedings of the International Workshop on Environmental Management, Science and Engineering - Volume 1: IWEMSE, ISBN 978-989-758-344-5, pages 517-523. DOI: 10.5220/0007562605170523


in Bibtex Style

@conference{iwemse18,
author={Y. Zou and X. C. Xu and X. J. Wang and F. L. Yang and S. S. Zhang},
title={Optimization of Culture Conditions for Chlorella Vulgaris Using Corresponding Normal Surface and Determination of Coefficients for Batch Kinetics of Nitrogen Removal Rate},
booktitle={Proceedings of the International Workshop on Environmental Management, Science and Engineering - Volume 1: IWEMSE,},
year={2018},
pages={517-523},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007562605170523},
isbn={978-989-758-344-5},
}


in EndNote Style

TY - CONF

JO - Proceedings of the International Workshop on Environmental Management, Science and Engineering - Volume 1: IWEMSE,
TI - Optimization of Culture Conditions for Chlorella Vulgaris Using Corresponding Normal Surface and Determination of Coefficients for Batch Kinetics of Nitrogen Removal Rate
SN - 978-989-758-344-5
AU - Zou Y.
AU - Xu X.
AU - Wang X.
AU - Yang F.
AU - Zhang S.
PY - 2018
SP - 517
EP - 523
DO - 10.5220/0007562605170523