Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)

Fitri Yuniarti, Wahyu Hidayati, Lulu Shofaya

Abstract

Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic bacteria producing secondary metabolites. Several studies have reported that secondary metabolite compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion method and identification of molecular isolates which had the highest antibacterial activity. After isolation, three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test showed that BW1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which have antibacterial activity against Bacillus subtilis and Salmonella typhi.

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Paper Citation


in Harvard Style

Yuniarti F., Hidayati W. and Shofaya L. (2018). Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).In Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development - Volume 1: MICH-PhD, ISBN 978-989-758-349-0, pages 169-175. DOI: 10.5220/0008241201690175


in Bibtex Style

@conference{mich-phd18,
author={Fitri Yuniarti and Wahyu Hidayati and Lulu Shofaya},
title={Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)},
booktitle={Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development - Volume 1: MICH-PhD,},
year={2018},
pages={169-175},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008241201690175},
isbn={978-989-758-349-0},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development - Volume 1: MICH-PhD,
TI - Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)
SN - 978-989-758-349-0
AU - Yuniarti F.
AU - Hidayati W.
AU - Shofaya L.
PY - 2018
SP - 169
EP - 175
DO - 10.5220/0008241201690175