Amoebicidal Activities of Indonesian Medicinal Plants in Balikpapan, East Kalimantan

Fendi Yoga Wardana, Defi Kartika Sari, Myrna Adianti, Adita Ayu Permanasari, Lidya Tumewu, Tomoyoshi Nozaki, Aty Widyawaruyanti, Achmad Fuad Hafid

Abstract

Entamoeba histolytica is a protozoan agent causing human amoebiasis, which is responsible for 100,000 deaths annually throughout the world. The recommendation in the treatment of amoebiasis using metronidazole has been reported to be less effective, because of the drug resistance effect by Entamoeba histolytica. Therefore, the search of new drugs with amoebicidal activity is important. The natural substances from medicinal plants are potentially a good object to be studied. The aim of this study was to evaluate Indonesian medicinal plants for their anti- amoebic activities. The hexane, dichloromethane and methanol extracts of 114 samples derived from 22 species of medicinal plants explored in the Balikpapan forest, East Kalimantan had been tested. Their anti-amoebic activity was determined by in vitro cell-based assay against Entamoeba histolytica HM-1:IMSS (clone 6) strain. According to cell-based assay, five of 114 samples tested showed anti-amoebic activities. The highest anti-amoebic activity was obtained from the dichloromethane extract of Cratoxylum sumatranum stembark with 50% inhibitory concentration (IC 50 ) of 22.07 ± 0.84 μg/ml. Subsequently, the dichloromethane extract of leaves and the dichloromethane extract of stem from Garcinia parviflora with IC 50 of 38.59 ± 9.46 μg/ml and 68.34 ± 0.4 μg/ml, respectively. The hexane extract of stembark and the dichloromethane extract of stem from Cratoxylum sumatranum had IC 50 of 69.79 ± 16.58 μg/ml and 118.49 ± 15.26 μg/ml, respectively. The dichloromethane extracts of Cratoxylum sumatranum stembark and Garcinia parviflora leaves are the most potential candidates in the development of anti-amoebic drugs.

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Paper Citation


in Harvard Style

Wardana F., Sari D., Adianti M., Permanasari A., Tumewu L., Nozaki T., Widyawaruyanti A. and Hafid A. (2018). Amoebicidal Activities of Indonesian Medicinal Plants in Balikpapan, East Kalimantan.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 77-82. DOI: 10.5220/0008357700770082


in Bibtex Style

@conference{bromo18,
author={Fendi Yoga Wardana and Defi Kartika Sari and Myrna Adianti and Adita Ayu Permanasari and Lidya Tumewu and Tomoyoshi Nozaki and Aty Widyawaruyanti and Achmad Fuad Hafid},
title={Amoebicidal Activities of Indonesian Medicinal Plants in Balikpapan, East Kalimantan},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={77-82},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008357700770082},
isbn={978-989-758-347-6},
}


in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Amoebicidal Activities of Indonesian Medicinal Plants in Balikpapan, East Kalimantan
SN - 978-989-758-347-6
AU - Wardana F.
AU - Sari D.
AU - Adianti M.
AU - Permanasari A.
AU - Tumewu L.
AU - Nozaki T.
AU - Widyawaruyanti A.
AU - Hafid A.
PY - 2018
SP - 77
EP - 82
DO - 10.5220/0008357700770082