Antidiabetic Activity of Diterpene Lactone Fraction of "Sambiloto" (Andrographis paniculata Nees.) on Mice (Mus musculus) Induced by Alloxan

Rani Maylana, Wiwied Ekasari, Lusiana Arifianti, Hadi Poerwono, Sukardiman

Abstract

Objective: The present study was carried out to evaluate the antidiabetic activity of diterpene lactone fraction of “Sambiloto” (A. paniculata Nees.) Methods: Antidiabetic was induced experimentally by a single intraperitoneal administration of 3.8 mg/20 g body weight of Alloxan-monohydrat in each male mice. Three days after alloxan administration, blood glucose level was measured using a glucometer. Mice with blood glucose levels above 200 mg/dL were considered diabetic and were used in this study. Then the mice were randomly divided into five experimental groups of six mice each. First group as positive control (Glibenclamide 0.06 mg/20 g body weight). Second group as negative control (CMC-Na 0.5%). Samples were given by oral route at three dosage levels (7.4 mg, 14.9 mg, and 22.3 mg/20 g body weight). After treatment finished, blood sampling was done by sterilizing the tail with alcohol and then nipping the tail at the start of the experiment and this was repeated after 2nd, 4th, 6th, and 24th h. Results: Results were expressed as Mean±SEM of the blood glucose levels per number of animals used in every study point. One-way ANOVA and post-ANOVA (LSD) test were used to compare the means in each group. Conclusion: Diterpene lactone fraction of “Sambiloto” had antidiabetic activity and the most effective dose was at 14.87 mg/20 g body weight of mice.

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in Harvard Style

Maylana R., Ekasari W., Arifianti L., Poerwono H. and Sukardiman. (2018). Antidiabetic Activity of Diterpene Lactone Fraction of "Sambiloto" (Andrographis paniculata Nees.) on Mice (Mus musculus) Induced by Alloxan.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 277-280. DOI: 10.5220/0008361202770280


in Bibtex Style

@conference{bromo18,
author={Rani Maylana and Wiwied Ekasari and Lusiana Arifianti and Hadi Poerwono and Sukardiman},
title={Antidiabetic Activity of Diterpene Lactone Fraction of "Sambiloto" (Andrographis paniculata Nees.) on Mice (Mus musculus) Induced by Alloxan},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={277-280},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008361202770280},
isbn={978-989-758-347-6},
}


in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Antidiabetic Activity of Diterpene Lactone Fraction of "Sambiloto" (Andrographis paniculata Nees.) on Mice (Mus musculus) Induced by Alloxan
SN - 978-989-758-347-6
AU - Maylana R.
AU - Ekasari W.
AU - Arifianti L.
AU - Poerwono H.
AU - Sukardiman.
PY - 2018
SP - 277
EP - 280
DO - 10.5220/0008361202770280